Why is YWHAH analysis important for my research?

YWHAH, a member of the 14-3-3 protein family, is a critical signaling molecule involved in diverse cellular processes. Aberrant expression or function of YWHAH has been linked to a wide range of pathologies, including cancers and neurodegenerative and autoimmune diseases, making its analysis essential for understanding disease mechanisms and discovering novel biomarkers.

How can I choose the right YWHAH analysis service for my project?

Selecting the appropriate analysis depends on your research questions. If you are interested in gene regulation, our gene expression and polymorphism services would be suitable. If your focus is on protein function or as a circulating biomarker, our immunoassay or proteomic profiling services are the best options.

Can you help me with a YWHAH assay for a non-human species?

Yes, we can assist with YWHAH analysis for a variety of species. Given the highly conserved nature of the 14-3-3 protein family, we can develop and optimize species-specific assays. We will work with you to ensure our methods are validated for your particular non-human model.

How do you ensure the specificity of your YWHAH antibodies?

We ensure the specificity of our YWHAH-specific antibodies through rigorous validation. This includes testing them against other members of the 14-3-3 family and using techniques such as WB with knockout or knockdown cell lines to confirm they only bind to YWHAH and not to other related proteins.

Do you offer services for detecting YWHAH in extracellular vesicles (EVs)?

Yes, we can perform analysis for the detection of YWHAH in extracellular vesicles. This is an emerging area of research, and we have the expertise to isolate and analyze EV-derived proteins.

What is the minimum sample volume required for your immunoassays?

The minimum sample volume required for our immunoassays will vary based on the specific assay format and the expected concentration of YWHAH in your sample. We will discuss these requirements with you during the initial project planning phase to ensure your samples are sufficient for the analysis.

YWHAH Analysis Service - Creative Biolabs

Creative Biolabs is your trusted partner for custom YWHAH analysis, providing a variety of strategic options to help you achieve your research objectives. We are well-equipped to assist with customized assay development, validation, and comprehensive solutions for your biomarker analysis needs. Our integrated biomarker analysis systems offer reliable, rapid, and cost-effective YWHAH analysis services for researchers worldwide.

Introduction of YWHAH

YWHAH (14-3-3η or 14-3-3 protein eta), an acidic protein primarily found in the brain, plays a critical role in neural tissue differentiation and early mesenchyme. As a member of the highly conserved 14-3-3 protein family, it functions in monoamine biosynthesis. YWHAH regulates the stability of human tyrosine hydroxylase type 1 (hTH1), negatively regulates parkin's ubiquitin ligase activity, and stimulates the activity of glutamate decarboxylase 1 (GAD1) to catalyze GABA formation.

Fig.1 Structure of YWHAH.

Genetic variations in the YWHAH gene, such as changes in a 7 bp repeat in its 5' UTR, have been associated with early-onset schizophrenia and psychotic bipolar disorder. YWHAH mutations have also been linked to early-onset epileptic encephalopathy, underscoring its role in neurological health. In cancer, YWHAH is a target of miR-660-5p. Its knockdown partially reverses the tumor-suppressive function of the miR-660-5p inhibitor. This miR-660-5p/YWHAH axis activates the PI3K/AKT pathway, promoting EMT and cell cycle progression.

YWHAH Analysis Services at Creative Biolabs

At Creative Biolabs, our YWHAH analysis services provide a comprehensive, modular approach to YWHAH biomarker research. With expertise in recombinant antibody expression, we have developed and validated highly specific YWHAH antibodies for various scientific applications like WB, IHC, ICC, ELISA, and FCM. Leveraging our state-of-the-art resources, we offer a powerful suite of analytical solutions tailored to your specific project needs, including but not limited to:

We provide precise determination of YWHAH gene expression using qPCR, which is essential for investigating transcriptional regulation and understanding YWHAH's function at the genetic level.

Our advanced genotyping assays enable a detailed assessment of YWHAH gene polymorphisms. This service helps identify genetic variations linked to disease susceptibility or drug response.

Our highly sensitive immunoassays enable the in vitro quantification of YWHAH protein concentration in diverse samples. This is crucial for evaluating YWHAH's role in disease prognosis and therapeutic efficacy.

Service Workflow

Our streamlined service workflow is designed for efficiency and precision, ensuring a seamless experience from initial consultation to final report delivery.

01Consultation and Project Planning

We begin with a detailed consultation to understand your specific research goals, project scope, and sample types. This step is crucial for customizing the workflow to your needs.

02Sample Submission

You will provide the necessary biological samples or cell lines. Our team provides clear guidelines for proper sample handling and shipping to maintain sample integrity.

03Laboratory Analysis

Our expert team proceeds with the designated YWHAH analysis, which may involve qPCR for gene expression, genotyping for polymorphisms, or immunoassays for protein quantification. All procedures are carried out under strict quality control standards.

04Data Processing and Analysis

After the assays are completed, we process the raw data and perform a professional analysis. Our bioinformatics specialists ensure the data is accurate and ready for interpretation.

05Final Report Delivery

Upon completion, we deliver a comprehensive final report. This document includes all raw data, detailed methodologies, and a professional analysis of the results to help you understand the significance of your findings.

Applications

Biomarker Discovery and Validation

Precise quantification of YWHAH protein and gene expression can help identify and validate YWHAH as a novel biomarker for a range of diseases, particularly in early-stage diagnosis of inflammatory conditions like rheumatoid arthritis and neurological disorders.

Disease Prognosis and Monitoring

By measuring changes in YWHAH levels over time, researchers can use our analysis services to monitor disease progression and evaluate the effectiveness of therapeutic interventions. This is especially relevant in conditions where YWHAH has been shown to be a prognostic indicator.

Pathway and Functional Studies

Our services, including assays for gene expression and protein levels, allow researchers to investigate the role of YWHAH in complex cellular signaling pathways, such as the PI3K/AKT pathway in cancer. This helps to elucidate the underlying molecular mechanisms of disease.

Drug Discovery and Development

YWHAH's involvement in multiple diseases makes it a promising therapeutic target. Our analysis services can be used to screen for compounds that modulate YWHAH activity or expression, accelerating the drug discovery process.

Service Highlights

FAQs

Creative Biolabs supports researchers to efficiently identify and analyze molecular biomarkers. Our biomarker analysis services are highly customizable according to the specific requirements of the customers. If you are interested in our analysis services, please feel free to contact us for more information.