Creative Biolabs has sufficient expertise in the development, production, and distribution of rodent disease models. Particularly, we can provide 5/6 nephrectomy model induced in both rats and mice for various investigation objectives.
Chronic renal failure (CRF) is a serious clinical problem and currently, there are no adequate therapeutic strategies for treatment. Many possible treatment strategies have been tested in rats with CRF induced by subtotal nephrectomy. Subtotal nephrectomy, so-called 5/6 nephrectomy, is probably the most established method to mimic the progressive renal failure after the loss of renal mass in humans. The predominant pathological abnormalities are glomerulosclerosis and tubulointerstitial fibrosis. There are two very different models encompassed: the ligation model and the ablation model.
Two types of 5/6 Nephrectomy
In the ligation model, branches of the renal artery in the rat are ligated after one kidney is removed (contralateral uninephrectomy), resulting in an obstruction of blood flow to the poles of the remaining kidney. Typically, 2/3 branches of the renal artery in the rat are ligated, while this approach cannot be performed in the mouse due to their limited renal artery branching. Besides, this infarction model is usually coupled with severe hypertension and more severe proteinuria.
In the ablation model, one kidney is removed first, and approximately 50% of the remaining kidney is removed by polar excision 1-2 weeks later. In this model, the rate of progression to renal failure is closely associated with the amount of tissue excised, therefore making it necessary to carefully calculate the proportion of excised tissue. This approach can be achieved in both rats and mice. A combination of ligation and ablation model is also feasible, which ties one or more branches of the mouse renal artery, and then cautery is performed as needed to remove additional renal mass to achieve a total 5/6 nephrectomy. In general, 129/Sv and Swiss-Webster mice can develop more significant glomerulosclerosis, proteinuria and additional features of sclerosis compared with C57BL/6 mice.
Fig.1 Infarction of 2/3 of the left kidney after
ligation of both polar arteries. (Iliescu et al.)
In brief, the partial nephrectomy model is of benefit to preclinical or research studies involving renal failure, hypertension or drug metabolism with impaired renal function. Creative Biolabs is capable of providing assessments for new therapies testing including but not limited to:
Meanwhile, Creative Biolabs also offers other types of rodent urological disease models that you may be interested in:
Creative Biolabs is known as a leading non-clinical CRO for preclinical drug development. We help to choose the most validated and reliable models for testing your compounds and our years of experience can help guide your decision regarding the best model for your application. Moreover, we work together with our clients to develop new animal models tailored to their specific pharmaceutical screening programs. If you are interested in any of these services, please don't hesitate to contact us for more details.
Reference
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