Antigen Specific T-Cell Recall Assay

Overview of Antigen-Specific T-Cell Recall Assay

Exposure of T lymphocytes to an antigen improves the immune response and leads to the generation of memory T cells that are antigen-specific and subsequently respond rapidly and aggressively when reexposed to the same antigen. Primary lymphocytes from tetanus vaccine recipients are frequently used to initiate an in vitro recall antigenic response. The development of an excellent antigen-specific T-cell recall assay platform will provide far-reaching significance for the immune-oncology field.

Fig.1 The circuit of anti-tumor T cell immunity. (Gitto, 2021)

Our Antigen-Specific T-Cell Recall Assay Service

Creative Biolabs provides one-stop antigen-specific T-cell recall assay services to support global customers. High-quality equipment, innovative technologies, and experienced scientists work together to make our services more flexible to meet customers' various needs.

In our antigen-specific T-cell recall assay service, T cells are antigenically activated after being co-cultured with antigen-presenting cells (APCs) or some special antigen peptides. To modify the activation response, antigen-stimulated T cells are cultured with some inhibitors of interest. Finally, endpoint detections were performed by various characterization experiments, which included lymphocyte proliferation, activation markers, cytokine production, and so on.

Fig.3 Workflow of antigen-specific T-cell recall assay. (Creative Biolabs)

Partnering With Us

Immune Cells with Strict QC to Empower Our Antigen-Specific T-cell Recall Assay Service

Our immune cells are obtained through an regulatory-approved donor program or IACUC protocol to aid antigen-specific T-cell recall assay services.

A Professional Cell Research Platform and Experimental Experts Enable Our Service More Reliable and Trustworthy

We have a professional technical team to support each project, at the same time, we have a strict quality control system from experimental design to the experimental process and result analysis, to ensure that the experiment is of high credibility.

One-Stop Service to Meet Customers' Various Needs

We provide one-stop services from sample processing to data visualization with the most up-to-date technical solutions.

Published Data

Here shows some literature data for reference.

2. Adenosine-related small molecules demonstrated the efficacy of recall antigen assay screening compounds for off-target effects on memory T cells

Fig.4 Adenosine-based recall antigen assay. (Kleiman, 2021)

Frequently Asked Questions

Q: Whether there are individual differences in the use of cells from different donors for antigen-specific T-Cell recall assay?

A: We believe that there is, and in this case, we would advise our customers to focus on the overall trend of the final test results rather than limiting them to a single data point.

If you are interested in our antigen-specific T-cell recall assay service, please don't hesitate to contact us for more information.

References

Gitto, Silvia, et al. "The emerging interplay between recirculating and tissue-resident memory T cells in cancer immunity: lessons learned from PD-1/PD-L1 blockade therapy and remaining gaps." Frontiers in Immunology 12 (2021): 755304.
Distributed under Open Access License CC BY 4.0, without modification.
Kleiman, Eden, et al. "Adenosine-related small molecules show utility of recall antigen assay to screen compounds for off-target effects on memory T cells." Scientific Reports 11.1 (2021): 9561.
Distributed under Open Access License CC BY 4.0, without modification.