Cassette dosing is an efficient technique mainly applied in drug discovery efforts of pre-clinical studies, in order to obtain pharmacokinetic data from multiple candidate drugs in a single experiment. Thus, a similar amount of information obtained from 5-10 separate PK studies can be available from a single study. Cassette dosing studies can facilitate rapid evaluation of multiple compounds with a small number of animals. As a world leader in the industry of drug discovery, Creative Biolabs now provides cassette dosing studies for pharmacokinetic assays, in order to accelerate the speed of drug screening. Our scientists will spare no effort to assist you in formulating a most efficient and cost-effective proposal for your drug discovery projects.

Cassette Dosing Studies

Cassette dosing, alternatively called N-in-1 dosing, is an attractive procedure for higher-throughput screening in drug discovery to rapidly screen large numbers of candidate compounds for assessing their pharmacokinetic properties, while minimizing the number of animals. In a cassette dosing procure, multiple candidate compounds are administered simultaneously to a single animal. The plasma samples obtained from blood samples are analyzed through an assay method such as liquid chromatography coupled to tandem mass spectrometry, which enables simultaneously quantitative assays of several compounds in a single sample. Accordingly, the pharmacokinetics of multiple candidate compounds can be assessed rapidly in a relatively shortened assay time, and with a small number of experimental animals. On the other hand, co-administration of multiple compounds might cause pharmacokinetic drug-drug interactions.

Conventional dosing versus cassette dosing in the pharmacokinetic screening of five compounds. Fig.1. Conventional dosing versus cassette dosing in the pharmacokinetic screening of five compounds. (Manitpisitkul and White, 2004)

Advantages

Compared with discrete dosing, advantages of cassette dosing include:

  • A reduced number of studies and animals required to support lead optimization;
  • A decreased inter-individual variability in comparison of compounds;
  • The generation of fewer samples for quantitative analysis.

Consequently, cassette dosing may improve the efficiency and productivity of the industrial pharmacokinetic assay in drug discovery. This methodology has been regarded as a helpful technique for assessing the PK properties of a series of compounds during early drug discovery efforts.

What We Offer

Creative Biolabs is committed to offering expert high-quality contract research services, especially in vivo pharmacokinetic assays. A typical cassette dosing study in Creative Biolabs commonly involves simultaneous administration of 5-10 compounds to a group of animals. These studies are commonly conducted in mice or rats, and a single bolus dose is given either i.v. or p.o. route. Serial blood samples will be collected, and LC-MS/MS techniques will be used to measure the concentration of each analyte in each sample. Moreover, according to your special requirements in cassette dosing study design, our experienced scientists are proud to offer customized services with fast turnaround time, which will help you reducing your total study costs and saving your time.

Appropriate application of in vivo pharmacokinetic study and assay method can significantly accelerate drug discovery and development. Creative Biolabs now offers a set of in vivo pharmacokinetic assay services in addition to cassette dosing studies, including:

Besides, you might be also interested in other in vivo PK studies listed below:

If you have any special needs in in vivo pharmacokinetic assays or be interested in learning more about Creative Biolabs’ pharmacokinetic assay services, please contact us for more information.

Reference

  1. Manitpisitkul, P.; White, R. E. Whatever happened to cassette-dosing pharmacokinetics? Drug Discovery Today. 2004, 9(15): 652-8.

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