Creative Biolabs is one of the most reliable industry leaders professional in developing animal models of human diseases. We provide chemical-induced rodent contact hypersensitivity (CHS) models established with different types of sensitizers, sensitization/challenge schedules and mouse strains for pre-clinical research as well as pathogenesis studies of human allergic contact dermatitis.

Chemical-Induced Rodent Contact Hypersensitivity (CHS) Models

Allergic contact dermatitis (ACD) is a common T-cell mediated skin inflammatory disease in humans. It was caused by repeated skin exposure to contact allergens and is characterized by redness, papule, and vesicles, followed by scaling and dryness. Pathophysiological understanding of ACD is derived from animal models referred to as contact hypersensitivity (CHS), in which the skin inflammation is induced by painting a hapten onto the skin. Commonly used dermal sensitizers to stimulate an acute CHS typically include 2,4,6-trinitrochlorobenzene (TNCB), oxazolone, fluorescein isothiocyanate (FITC) and 2,4-dinitrofluorobenzene (DNFB). These models share many of the features of human allergic contact dermatitis. Specifically, a list of CHS models has been established at Creative Biolabs using different sensitizers, sensitization/challenge schedules and mouse strains.

Description of Chemical-Induced CHS Models

Rodent CHS models are easy to handle, highly reproducible, inexpensive and well characterized. Generally, two distinct phases are necessary to achieve optimal CHS reaction. One is the sensitization phase, also known as afferent or induction phase, which starts with the first contact of skin with hapten. The other is challenge phase, also known as efferent or elicitation phase, which occurs following second hapten contact. While sensitization phase has no clinical manifestations in the majority of cases, challenge of the skin of sensitized individuals leads to local inflammatory reaction.

Pathophysiology of allergic contact dermatitis.Fig.1 Pathophysiology of allergic contact dermatitis. (Elliott et al. 2010)

During the sensitization phase, the hapten triggers danger signals and the release of inflammatory cytokines and is taken up by activated dendritic cells, which migrate to the skin-draining lymph nodes and prime naive T cells. In the elicitation phase, upon re-exposure to the hapten, the allergen-specific T cells are recruited into the skin. The disease becomes manifest because of the cytotoxicity of CD8+ effector T cells and the interaction of recruited inflammatory cells with keratinocytes and stromal cells.

Creative Biolabs provides such assessments as:

  • PK/PD Blood Collections
  • Clinical Score/Ear Thickness
  • Biomarker Analysis
  • Histopathologic Evaluation
  • Immunohistochemistry

Meanwhile, in order to meet our customers' specific requirements and their various research objectives, Creative Biolabs also offers other rodent inflammatory & immunological disease models listed as follows that you might be interested in:

Creative Biolabs is a leading-industry with high-end technology platforms and seasoned research teams. We are proud to offer cost-effective studies with high-quality and competitive prices. Contact us if you need more information or a formal quote.

Reference

  1. Elliott, G.; Das, P.K. Defining the antigen determinant for T-cell-mediated contact dermatitis using p-phenylenediamine: a gateway to chemical immunology[J]. Journal of Investigative Dermatology. 2010, 130(3):641-3.

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