As a leading provider of drug development services, Creative Biolabs is capable of providing different kinds of rodent IBD models for preclinical screening, testing, and evaluation of drug candidates. These include DSS-induced acute and chronic colitis models, TNBS/DNBS-induced colitis models, oxazolone-induced colitis models, and indomethacin-induced colitis models.

Introduction of Inflammatory Bowel Disease

Inflammatory Bowel Disease (IBD), a heterogeneous group of disorders characterized by chronic, uncontrolled inflammation in the gastrointestinal (GI) tract, has been a worldwide health-care problem with a continually increasing incidence. Two major phenotypic forms, Crohn's Disease (CD) and Ulcerative Colitis (UC) are encompassed, both displaying various symptoms such as loss of body weight, abdominal pain, and diarrhea with blood. They are classified according to the location of the GI tract being affected: UC mainly affects the colon and rectum, while CD, can affect any part of the gastrointestinal tract, although ileum and colon are most frequently involved. Although the etiopathogenesis is poorly defined, it is revealed by current IBD models that disease stems from interactions between genetically susceptible hosts and the gut microflora.

Pathogenesis of DSS-Induced Colitis

Dextran sodium sulfate (DSS), a water-soluble, negatively charged sulfated polysaccharide containing ~17% sulfur with a highly variable molecular weight ranging from 5 to 1,400 kDa, is often used to induce a form of mouse colitis that mimics the clinical and histological features of IBDs that have characteristics of UC. The symptom severity of induced murine colitis depends on not only mouse strain, mouse gender but also the dose of chemical inducers and other various elements. The current hypothesis of how colitis-inducing injury occurs is that DSS can damage the mucosal membrane of the colon allowing the dissemination of pro-inflammatory intestinal contents (e.g. bacteria and their products) into the crypt. The DSS colitis model is widely used in IBD research due to its rapidity, simplicity, reproducibility, and controllability.

Dextran Sulfate Sodium (DSS)-Induced Rodent Colitis ModelFig.1 The disease activity index of DSS-induced colitis in mice is reduced with treatment of Infliximab and Magnolol. (Zhao et al. 2017)

Colitis Induction with DSS

Particularly, acute, chronic and relapsing models of intestinal inflammation can be achieved by modifying the concentration of DSS and the frequency of administration. Colitis is established by the oral administration of DSS via drinking water, which will lead to inflammation in the mid-distal colon. This leads to decreased body weight, bloody diarrhea, and eventually death in a time- and dose-dependent manner. For evaluation of potential anti-inflammatory drug candidates, Creative Biolabs provides various assessments including but not limited to:

  • Body weight
  • Clinical score
  • Stool consistency and hemoccult positivity
  • DIA (Disease Activity Index)
  • Colon length & weight
  • Histology & immunohistochemistry
  • Intestinal bleeding and permeability
  • Epithelial cell proliferation and migration
  • Myeloperoxidase (MPO) assay
  • Endoscopy

Meanwhile, Creative Biolabs also offers other types of rodent digestive system disease models that you may be interested in. They are listed as follows for you to review:

Creative Biolabs offers CRO services with extensive expertise in conducting preclinical drug development, proof-of-concept and mechanism of action studies in various rodent models. Our team has the skills and experience required to evaluate your preclinical drug candidates for efficacy studies, in vivo pharmacology studies and so on. Moreover, professional interpretation of the preclinical data is also provided to facilitate your drug development.

Contact us for more information or a formal quote, if you are interested in any of these services.

Reference

  1. Zhao, L.; et al. Magnolol, a Natural Polyphenol, Attenuates Dextran Sulfate Sodium-Induced Colitis in Mice[J]. Molecules. 2017, 22(7):1218.

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