For testing or evaluating anti-peptic ulcer activity of potential drug candidates, many rodent models have been applied including NASAIDs-induced gastric ulcer models, ethanol-induced gastric ulcer models and so on. At Creative Biolabs, we offer ethanol-induced gastric ulcer models induced in both rats and mice.
Introduction of Peptic Ulcer
Peptic ulcer is among the most predominant gastrointestinal diseases in the world and is characterized by ulceration in the stomach and duodenum. Generally, the pathophysiology of ulcer is caused by an imbalance between the gastric aggressive factors (e.g., acid, pepsin, Helicobacter pylori infection, and non-steroidal anti-inflammatory agents) and the mucosal defensive factors such as mucus bicarbonate and blood flow. Although several orthodox drugs are available for the treatment of the disease, there has been a growing interest in the development of alternative therapies due to the adverse effects associated with the existing drugs which, in turn, limit their use.
The Mechanisms of Ethanol-induced Gastric Ulcers
As a potential risk factor of gastric ulcer development, ethanol is capable of penetrating the gastric mucosa resulting from the solubilization of the protective mucous, and then exposing the mucosa to the proteolytic and hydrolytic actions of hydrochloric acid and pepsin, which will subsequently lead to membrane injury. What's more, ethanol can stimulate acid secretion, reduce blood flow via disruption of vascular endothelium, and trigger the release of superoxide anion leading to an increased oxidative stress in the tissues. Using ethanol as the inducer, the experimental model has exhibited symptoms that resemble acute peptic ulcers in humans and is suitable and useful for studying the efficacy of potential therapeutics that has cytoprotective and antioxidant activities.
Fig.1 Macroscopical appearance of the gastric mucosa in rats. (A)The negative control group has no injury of gastric mucosa. (B) The ulcer control group displays severe injuries in the gastric mucosa. (Hajrezaie et al. 2015)
Our Capabilities
Creative Biolabs has sufficient experience in conducting in vivo preclinical evaluation of potential new therapeutics. Our capacity is robust for the evaluation of a wide range of biologics and small molecules including synthetic organic compounds and non-protein natural products. Moreover, a variety of administration routes and clinically relevant endpoints are available to tailor your digestive discovery projects.
Induction of Gastric Ulcers Using Ethanol
Prior to the induction, animals are generally fasted for 24-36 h, followed by the administration of absolute ethanol. One hour later, the animals can be sacrificed and the assessments can be determined. At our preclinical center, we provide histopathological analysis including H&E and PAS (Periodic acid Schiff) staining for the evaluation of stomach injuries. MDA is measured to assess the oxidative stress. Biomarkers of necrosis, apoptosis, and stress response are analyzed by immunohistochemistry.
Assessments
We provide assessments including but not limited to:
Meanwhile, Creative Biolabs also offers other types of rodent digestive system disease models such as:
As a leading CRO research partner for drug development, Creative Biolabs is committed to providing rapidly scheduled, accurate and cost-effective services to our clients. Moreover, we offer customized solutions by working with our clients to develop study-specific experimental protocols as well as novel models. If you're interested in our services, please contact us to discuss your specific requirements.
Reference
For Research Use Only.
