In recent years, therapeutic antibodies have come to light and brought good news to the treatment of autoimmune diseases, cancers, inflammatory diseases, and many other types of diseases. Dedicated to antibody research and development for decades of years, Creative Biolabs is a leading service provider that focuses on antibody engineering. Our professional academic teams are confident in applying our experience, industry and regulatory knowledge to offer high-quality custom-specific antibody Fc engineering services to clients globally.
Fc region is a crystallizable fragment of an antibody that located at the base of the "Y", which is responsible for binding to Fc receptors. The Fc region consists of two heavy chains that contribute two or three constant domains depending on the isotype of the antibody. There are five different types of Fc regions in general, which allow antibodies to be grouped into five isotypes that are IgG, IgM, IgA, IgD, and IgE. Exception for IgD, each Fc region of a certain antibody isotype binds to corresponding Fc receptor and enables the antigen-antibody complex to exert different roles depending on which Fc receptor it binds.
By binding to specific Fc receptors and other immune molecules, the Fc region ensures that a specific antibody generates an appropriate immune response for a different antigen, and thus mediating different physiological effects and effector functions, including but not limited to:
Fig.1 Crystal structures illustrating Fc interactions with FcγRs and FcRn. (Saxena, 2016)
Given the essential roles that the Fc region plays in the modulation of the efficacy of mAb in different disease treatments, Fc engineering has been extensively studied in the past years. To meet different research demands, e.g., prolonging antibody serum half-life, increasing or decreasing effector functions, Creative Biolabs is now offering a comprehensive portfolio of Fc engineering services to modulate antibody characteristics and functions based on our advanced technology platforms and abundant experience. Different strategies for antibody Fc engineering provided by Creative Biolabs include:
Skeleton engineering can be achieved by amino acid substitution in the FcγR binding site with the antibody’s Fc domain and exchanging larger amino acid stretches between different isotypes. Consequently, variants with altered FcγR binding profiles can be generated and selected for optimal characteristics.
IgG antibodies contain a conserved N-glycosylation site on the Fc domain to which a complex, biantennary glycan is attached. The fine structures of this glycan modulate antibody effector functions by affecting the binding affinity of the Fc to Fc receptors. Moreover, glycosylation also plays a vital role in antibody drug properties, including immunogenicity and half-life. Creative Biolabs offers an integrated approach to control the composition of glycans, thereby modulating the safety and efficacy of antibody therapeutics. Services provided here include:
As a leading service provider of antibody research, Creative Biolabs provides comprehensive Fc engineering services based on plenty of advantages which including but not limited to:
We are pleased to use our extensive experience and advanced platforms to offer the best service and the most qualified products to satisfy each demand from our customers. For more information, please contact us or send us an inquiry directly.
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