Creative Biolabs has extensive experience in establishing chemically induced diabetic mice/rats models for our global customers, especially for the high fat diet/STZ induced diabetic model.

High Fat Diet/STZ Induced Diabetic Model Fig.1 Characteristics of Type 2 Diabetes

Type 2 Diabetes

Type 2 diabetes is a long-term metabolic disorder characterized by high blood sugar, insulin resistance and relative lack of insulin. Now there has been a tragic increase in diabetes worldwide, paralleling the overweight and obesity epidemic. In order to further elucidate the pathobiology and to find better treatments and novel prevention strategies for this complex disease, appropriate experimental animal models are of great value. One example of type 2 diabetes animal models is the high fat diet-fed, streptozotocin (HFD/STZ)-treated rat model.

High Fat Diet/STZ Rat Model

Rats fed with high-fat diet are useful models to mimic insulin resistance, which is one of the important features of type 2 diabetes. At the same time, high-dose streptozotocin (STZ) is widely used to induce type 1 diabetes in adult mice, resulting from the β cell death through alkylation of DNA. However, low-dose STZ has been shown to induce a mild impairment of insulin secretion, which is similar to the feature of the later stage of type 2 diabetes. Based on the above two points, investigators have started to develop a new diabetic model by the combination of diet (high fat or high fructose diet) plus the treatment of STZ.

In the first place, rats are fed with above special diets, which initially produces hyperinsulinemia and insulin resistance. Subsequently, these sensitive subjects are treated with low-dose STZ that causes the β cell damage and frank hyperglycemia.

Therefore, by feeding the animal with high-fat diet following low-dose STZ, a useful type 2 diabetic rat model that would closely mimic the natural history and progression of the disease events (from insulin resistance to β cell dysfunction) as well as metabolic characteristics of human type 2 diabetes is established. Compared to other models, this model is cheaper, easily accessible, and practical for the investigation as well as testing of potential novel therapeutics for the treatment of type 2 diabetes.

High Fat Diet/STZ Induced Diabetic ModelFig.2 Effect of high-fat diet or high-fat diet and low-dose streptozotocin on glucose tolerance. (Davidson et al. 2014)

Creative Biolabs provides various assessment options for testing the effectiveness of potential antidiabetic agents depending on your study objectives, which including but not limited to:

  • Body Weight
  • Histopathological Evaluation
  • Blood Glucose Level
  • Food/Water Intake
  • Fasting Blood Glucose
  • Serum Insulin Level
  • Biomarkers

Additionally, Creative Biolabs also offers other types of rodent metabolic disease models that you may be interested in:

If you are interested in any of these services, please contact us or send us an inquiry. We are willing to share our state-of-the-art platforms and sufficient expertise to boost your brilliant studies.


  1. Davidson, E. P.; et al. Differences and similarities in development of corneal nerve damage and peripheral neuropathy and in diet-induced obesity and type 2 diabetic rats. Investigative Ophthalmology & Visual Science. 2014, 55(3), 1222.

For lab research use only.

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