Drug-induced phospholipidosis (PLD) is a condition where certain drugs cause an accumulation of phospholipids in cells, particularly in organs like the liver, kidneys, and lungs. While phospholipidosis itself may not cause immediate harm, it can indicate potential toxicity and organ damage if left untreated. Therefore, it is crucial to find new treatments that can effectively address phospholipidosis and prevent further complications.
Fig. 1 Agents and factors causing lysosomal membrane permeabilization (LMP) leading to cell death.1
Improving phospholipidosis has attracted more attention from researchers. Significant efforts have been made to develop innovative solutions in this area. Creative Biolabs provides a comprehensive in vitro cell-based phospholipidosis assay service that assists clients in evaluating and profiling numerous chemicals for PLD.
Our cell-based assay is developed using fluorescent dye to treat cells in plates. By observing the fluorescence intensity change, we are able to quantify the PLD changes in cells and rapidly examine candidate compounds that can induce phospholipidosis. In addition, we also have the ability to conduct phospholipidosis assay through a gold-standard electron microscopy (EM) analysis or in animal models, although these methods need large amounts of compounds and take more days. We provide custom services to satisfy your specific demands. Importantly, Creative Biolabs stands as a dependable partner ready to propel your projects towards clinical deployment and ultimately to the market.
Fig. 2 Our in vitro cell-based phospholipidosis assay service process.
Fig.2 Our in vitro cell-based phospholipidosis assay service process.
Fluorescent Dyes at Creative Biolabs
Loss of BAZ2B function has been postulated to cause neurodevelopmental disorders, thus cause of developmental delays, intellectual disabilities, and autism spectrum disorders.
Fluorescent Dyes at Creative Biolabs
Loss of BAZ2B function has been postulated to cause neurodevelopmental disorders, thus cause of developmental delays, intellectual disabilities, and autism spectrum disorders.
Q1: How to measure the viability of cells after candidate compounds treatment?
A1: Detecting cell viability is crucial in various biological and pharmaceutical applications. Our advanced technologies, including fluorescence microscopy, flow cytometry, and microplate readers, offer accurate assessments of cell health. With specially designed reagents, these assays evaluate membrane integrity and cellular functions, providing essential insights into live, dead, and damaged cell populations. These tools can enhance research outcomes and support diverse scientific endeavors.
Are you seeking a reliable cell biology expert to move your phospholipidosis projects forward? Please feel free to get in touch with us for more details.
Reference
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