In Vitro Oris Cell Migration Assay

Creative Biolabs focuses on Oris assay for the migration of tumor cells to support our customers' scientific research in cancer and related biomedical industries.

In Vitro Oris Assay as a Readout for Migration of Tumor

Cell migration is a basic cellular behavior to maintain homeostasis. It is associated with angiogenesis to guarantee tumor nutrition and metastases formation. Improved high-throughput screening of migration methods will speed the discovery of potential lead therapeutic compounds.

Equipped with rich experience, Creative Biolabs provides a cutting-edge Oris assay service with the potential to aid in cancer research. We offer a full range of Oris cell migration assay-related services, including cell culture, cell line STR analysis, migration assay induction, and data analysis to suit our clients' cancer research and project development. In addition, we can establish cell lines and conditions upon customers' request.

Features & Benefits

Creative Biolabs is committed to offering efficiency, confidentiality, and rapid, accurate service with proven experience and expertise.

Workflow of Oris Cell Migration Assay

The Oris cell migration assay is based on a multi-step process, in which a mask is applied to the bottom of a 96-well microplate well first. Meanwhile, a polymeric insert (silicon stopper) is installed in the wells of the microplate. When cells are added, the insert prevents cells from seeding the internal analysis area. After cell attachment, the insert is removed, allowing cells to migrate freely to the central analysis area of the wells. Cells that do not migrate to the analysis area will not be observed due to the placement of the mask originally at the bottom of the plate. Migrating cells were analyzed by a microscopy or a synergy HT multimode reader with fluorescence.

Fig.1 The scheme of the Oris Cell Migration Assay process. (Creative Biolabs)

Published Data

• Paper Title: A Quantitative, Facile, and High-Throughput Image-Based Cell Migration Method Is a Robust Alternative to the Scratch Assay.
• Summary: The study performed an Oris cell migration test using an endothelial progenitor cell line and the Src kinase inhibitor dasatinib. They found that the Oris cell migration assay for cell migration analysis provides a powerful, efficient, economic method.
• Cell Type: Endothelial Progenitor Cells: ECFCs
• Assay Type: Oris Assay

Result:

Fig.2 Analysis of ECFC-covered area with the indicated concentration of dasatinib following stopper removal (pre-migration) or 24-h migration. (Gough, 2011)

Fig.3 Plate uniformity and dasatinib concentration-response results of ECFC migration. (Gough, 2011)

If you are interested in our Oris cell migration assay services, please contact us to discuss your project.

Reference

1. Gough, W.; Hulkower, KI.; et al. A quantitative, facile, and high-throughput image-based cell migration method is a robust alternative to the scratch assay. Journal of biomolecular screening. 2011, 16(2):155-63.

For Research Use Only.