Bradykinin receptors are a family of GPCRs and have two subtypes: B1R and B2R, mediating bradykinin signaling. Both are predominantly associated with phospholipase C activation and mediate the vascular aspects of inflammation. Clinical research shows that most efforts to synthesize potent bradykinin antagonists have focused on targeting the B2 bradykinin receptor. The search for B1R antagonists is also crucial because B1Rs are promising candidates for therapeutic development in pain processes. Consequently, identifying the function of bradykinin receptors will broaden their applications in drug development.
Fig.1 Bradykinin B2 receptor contributes to inflammatory responses in human endothelial cells.1
Magic™ in vitro cell-based bradykinin receptor functional assay service from Creative Biolabs is carried out via measuring the calcium flux and β-arrestin recruitment in response to cellular response triggered by the reaction of bradykinin receptors with their ligands. We provide a fast and effective Magic™ in vitro cell-based bradykinin receptor functional assay service covering from sample treatment to data visualization to meet global customers' diverse needs. Together with our seasoned and expert research teams, we are confident in presenting desirable results for every customer.
Fig.2 B2R heterodimers with AT1R, APJ, and κ-OR.2
Creative Biolabs has established a powerful technology platform and a strict SOP to provide global customers with reliable functional assay services. Here is a simplified workflow of our Magic™ in vitro cell-based bradykinin receptor functional assay service.
Fig.3 The workflow of our Magic™ in vitro cell-based bradykinin receptor functional assay service.
| Cell Bank System | Our stable expressing cell lines include human leukemia Jurkat T cells, human melanoma A375 cell lines, MCF-7 human breast cancer cells, CHO cell lines, etc., making them available for use in a wide variety of research projects by customers throughout the world. |
| Dependable Experiment Platforms | With years of project experience and ongoing optimization, our experimental system is at the cutting edge of today's technology. We are certain that by combining this with straightforward experimental methods, we will be able to provide the desired outcomes for customers all around the world. |
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Q1: What are the differences between bradykinin receptors B1 and B2?
A1: The bradykinin receptor has two isoforms: bradykinin type 1 and 2 receptors (B1R and B2R, respectively). B1R expression is modest in naive conditions and increases after inflammation. B2R, on the other hand, is constitutively expressed and does not vary in presentation in response to inflammation. Evidence shows that B2 receptors have a role in more traditional acute inflammatory events including edema and inflammatory pain. In contrast, B1 receptors seem to be engaged in chronic inflammatory responses like persistent hyperalgesia.
References
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