Magic™ In Vitro Cell-based Oxytocin Receptor Functional Assay Service

Oxytocin Receptors: Why They Matter?

The oxytocin receptor (OXTR) is a GPCR that specifically recognizes and responds to the neurotransmitter and hormone oxytocin. OXTR plays a crucial role in the peripheral control of parturition and breastfeeding, which has motivated several drug development initiatives to create agonists and antagonists for peripheral action. Modulating human social behaviors and connections, including cognition in mental or neurological illnesses, is another function of the oxytocin receptor. Due to this, agonists of this receptor show great potential for the treatment of many mental disorders. Therefore, comprehension of oxytocin receptors is of crucial importance.

Fig.1 Intranasal oxytocin: potential therapeutic regulation of brain function in psychiatric illness.¹

Our Magic™ In Vitro Cell-based Oxytocin Receptor Functional Assay Service

To respond to global customers' feedback, Creative Biolabs innovatively incorporates the cell-based assay with oxytocin receptors functional assay and succeeds in developing a Magic™ in vitro cell-based oxytocin receptor functional assay service featuring rapid and reliable.

Oxytocin receptors activate several second messenger systems. Leveraging this fact, our Magic™ in vitro cell-based oxytocin receptor functional assay service measures the accumulation of second messengers as an indicator in response to candidate samples' treatment. As a consequence, we may evaluate candidate samples for their usefulness and investigate the signaling pathway of oxytocin receptors. To ensure global customers' projects run smoothly, we provide comprehensive top-ranking customized services. Cordially welcome global customers to inquire for more details.

Fig.2 Oxytocin receptor analogs activate certain G protein subtypes.²

Benefits of Our Magic™ In Vitro Cell-based Oxytocin Receptor Functional Assay Service

Our Magic™ in vitro cell-based oxytocin receptor functional assay service overcomes the limitations of traditional oxytocin receptor functional assays, resulting in unique breakthroughs in technology, applications, and other areas. At the same time, our Magic™ in vitro cell-based oxytocin receptor functional assay service may provide considerable advantages to global customers.

Representative Data

Data 1: There is not sufficient evidence to demonstrate that the neurotransmitters oxytocin (OT) and 5-hydroxytryptamine (5-HT) interact via their respective receptors, especially the oxytocin receptor (OTR) and the 5-hydroxytryptamine 2C receptor (5-HTR2C), to regulate certain emotional behaviors. Here, utilizing flow cytometry-based FRET (fcFRET) and co-expression of them, the study provides evidence for a possible physical interaction between OTR and 5-HTR2C in an in vitro cellular expression system, verifying the results in mice. In summary, these results offer strong evidence for the development of active OTR/5-HTR2C heterocomplexes, which further complicate the signaling roles of OTR and 5-HTR2C.

Cell types: HEK293A cells

Fig.3 Co-expression of the OTR and 5-HTR2C.³

Data 2: Oxytocin-arginine vasopressin (OT-AVP) ligand receptors affect brain and peripheral physiological, behavioral, and social functions. Recent research has demonstrated that OT ligands and receptors in New World Monkeys (NWMs) are extraordinarily variable, providing a rare chance to investigate how genetic diversity affects the pharmacological fingerprints of peptide ligands. In this research, the downstream signaling changes triggered by arginine vasopressin and oxytocin analogs at different species were examined through Ca²⁺ mobilization assay and membrane potential assay in receptors expressing CHO cells. The study evaluated the pharmacological characteristics of oxytocin and arginine vasopressin analogs in marmosets, titi monkeys, macaques, and humans with receptors for oxytocin.

Cell types: Chinese hamster ovary (CHO) cell

Fig.4 Ca²⁺ mobilization induced by arginine vasopressin and oxytocin analogs.⁴

Work with Creative Biolabs

In light of the invaluable feedback we get from our global customers, Creative Biolabs is unwavering in its commitment to developing and introducing ground-breaking new technical innovations. If you are attracted by our Magic™ in vitro cell-based oxytocin receptor functional assay service, please feel free to contact us.

References

1. Macdonald, Kai, and David Feifel. "Helping oxytocin deliver: considerations in the development of oxytocin-based therapeutics for brain disorders." Frontiers in Neuroscience 7 (2013): 35.
2. Busnelli, Marta, and Bice Chini. "Molecular basis of oxytocin receptor signaling in the brain: what we know and what we need to know." Behavioral Pharmacology of Neuropeptides: Oxytocin (2018): 3-29.
3. Chruścicka, Barbara, et al. "Molecular, biochemical and behavioral evidence for a novel oxytocin receptor and serotonin 2C receptor heterocomplex." Neuropharmacology 183 (2021): 108394.
4. Pierce, Marsha L., et al. "Comparison of the pharmacologic profiles of arginine vasopressin and oxytocin analogs at marmoset, titi monkey, macaque, and human oxytocin receptors." Biomedicine & Pharmacotherapy 125 (2020): 109832.

For Research Use Only.