For compounds against intracellular targets, internalization ability is one crucial characteristic, as they will rely on this process to confer their intended effects. Particularly, antibodies with internalization potencies can also act as excellent vehicles for targeted delivery of drugs, toxins, enzymes or DNA into cells for various therapeutic/diagnostic applications, e.g. ADCs. Empowered by state-of-the-art techniques and rich experience, scientists in Creative Biolabs has established a versatile platform for internalization assessment. Here we are glad to introduce Magic™ pH-sensitive dye assay services for compound internalization profiling to worldwide researchers.
Introduction to Endocytosis
In the process of endocytosis, certain agents can transport themselves from extracellular environment into cytosol. Endocytosis can be classified into three categories: phagocytosis, pinocytosis, and receptor-mediated endocytosis. In phagocytosis, the cell' s plasma membrane wraps a macromolecule or even an entire cell and buds off to form a food vacuole or phagosome. In pinocytosis, the cell swallows drops of fluid by forming vesicles that are smaller than the phagosomes formed in phagocytosis. Both phagocytosis and pinocytosis are non-specific processes in which the cell takes in whatever solutes it envelops.
Fig. 1 Three types of endocytosis process.
Unlike phagocytosis and pinocytosis, receptor-mediated endocytosis is an extremely selective process of the cell. This specificity is mediated by receptors located on the cell membrane. Extracellular macromolecules (ligands) bind to particular cell surface receptors that are concentrated in clathrin-coated pits. These pits bud from the membrane to form small clathrin-coated vesicles wrapping the receptors and their bound macromolecules (ligands). The clathrin-coated vesicles then fuse with early endosomes, in which the contents are classified and transported to the lysosomes or recycled to the plasma membrane.
Magic™ pH Dye for Internalization Detection
Creative Biolabs now integrates a specialty pH-sensitive dye technique for internalization assays of diverse compounds. This particular dye is not fluorescent at neutral pH but will emit high fluorescence signals at acidic pH. As is known, there exists a clear difference in pH between body fluid (neutral) and cellular endosomes/lysosomal vesicles (acidic). Hence, this pH reactive dye conjugated to compound of interest can be used for dynamic detection of internalization activities, which can be easily monitored and analyzed using flow cytometry or fluorescent microscopy.
Unique Advantages of Magic™ pH Dye
Creative Biolabs now offers one-stop, custom-oriented internalization assay service using multiple strategies, from which you can always find the best match for your specific candidate. Please don't hesitate to contact us for more information.
Related Products:
Magic™ pH-Sensitive Dye Reagents
For Research Use Only.
