Creative Biolabs provides excellent services for the analysis and characterization of Fab antibody functions by our advanced antibody functional analysis platform. Our professional scientists can help global customers discover potential immune checkpoint functions. Particularly, we are happy to introduce our cost-effective mixed lymphocyte reaction (MLR) assay.
Mixed lymphocyte reaction (MLR) is a useful model used by pharmaceutical and biotech organizations to study alloresponsiveness to histocompatibility antigens. In short, it mixes populations of lymphocytes together and measures the reaction that occurs. Using the MLR, we can measure the disparity in major histocompatibility complex (MHC) antigens between individuals. Particularly, potential immunoactivity or immunosuppressive effects of your drugs can be determined using this assay.
Fig.1 Mixed lymphocyte reaction (MLR) tissue typing. (Mak, 2005)
To date, a few types of MLR have been developed to meet scientists’ guidelines. Among these methods, two main types of MLR, namely one-way MLR and two-way MLR, have been widely used to help researchers characterize their drug candidates’ potential ability to mount anti-tumor T cell responses in the tumor microenvironment. One-way MLR is a cell proliferation assay where one population of lymphocytes (responder cells) is stimulated to proliferate by another population of lymphocytes (stimulator cells, which have been rendered non-proliferative). On the contrary, the two-way MLR differs from the one-way MLR in that both of the cell populations are able to proliferate.
With abundant experience in this field, Creative Biolabs has established an advanced platform to help global researchers determine the effects of antibodies on the ability of T cells to be activated and proliferate. Depending on your wishes we can either offer one-way MLR or two-way MLR. Alternatively, the MLRs can be performed with various sources of antigen presenting cells (APCs), which include but are not limited to monocytes, immature and mature monocyte-derived dendritic cells (DCs), and plasmacytoid DCs. What’s more, our method will provide analysis and interpretation of the effects of your biologics or small molecules on multiple endpoints from multiple allogeneic donors.
Creative Biolabs has been devoted to the field of antibody function studies for over a decade and has established a series of technology platforms to integrate various antibody function analysis services. With extensive experience, abundant industry knowledge, and advanced technical skills, our scientists are fully confident in accomplishing even the most challenging projects to make your project a success. For more information, please feel free to contact us.
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