Hi-Affi™ Cellular Antioxidant Assay Kit (Green Fluorescence)-Fluorometric (CAT#: ZL-0324-WR44)

Datasheet
Overview
DescriptionThis kit offers a cell-based assay designed to measure the activity of an exogenous antioxidant compound within adherent cells.
FeaturesReady-used for antioxidant assay.
ApplicationsUseful for measuring antioxidant activity within adherent cells.
Research AreasOxidative stress; Cell Damage; Drug Discovery
Specification
Size192 tests
ComponentsThis kit is supplied with 96-well Cell Culture Microtiter Plates, DCFH-DA Probe, Free Radical Initiator, and Quercetin.
Target/DescriptionCells are initially grown in a 96-well black fluorescence cell culture plate until reaching confluency. Subsequently, the cells are treated with a cell-permeable DCFH-DA fluorescence probe dye and either the bioflavonoid Quercetin, or the antioxidant of interest. After a short incubation period, the cells are washed, and the reaction is initiated by adding a Free Radical Initiator. This compound generates free radicals that convert the probe into highly fluorescent DCF. Quercetin's impact on inhibiting free radical formation and subsequent DCF production is assessed in a dose-dependent manner.
StorageStore at the appropriate temperature as directed.
HandlingPlease handle the product at appropriate storage condition once received.
ShippingDry ice
Regulatory StatusRUO

For Research Use Only.

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