| Overview | |
|---|---|
| Description | This kit is designed for precise and accurate quantitative measurement of cathepsin E activity in various biological samples such as cell lysate, purified protein, and tissue lysate. |
| Features | Ready-used for protease assay |
| Applications | Kit is used to study the Cathepsin E protease in biological samples. |
| Application Notes | The kit provides a reliable and straightforward means for the quantification of Cathepsin E activity in tissue and cell lysates. |
| Molecular Mechanism of Action | The assay is based on the detection of protease activity using a fluorogenic peptide substrate that is specific for Cathepsin E. Upon cleavage of the substrate by Cathepsin E, a fluorescent signal is generated, which can be quantified using a fluorometer or fluorescence microplate reader at Ex/Em = 320/420 nm. |
| Assay Measures | Fluorogenic-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including CTSE assay buffer, CTSE lysis buffer, CTSE substrate, human cathepsin E (positive control) (50 µg), and MCA standard (1 mM). |
| Target | Cathepsin E |
| Species Reactivity | Mammals, Other species |
| Sensitivity | 1000 µU |
| Format | Supplied with fluorometric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
