The development and manufacture of therapeutic antibodies, including monoclonal antibodies (mAbs) and Fc fusion proteins, represent a significant segment of the biopharmaceutical industry due to their substantial benefits to public health. One of the key attributes of therapeutic antibodies is that they usually rely on the variable domain and Fc (crystallizable fragment) region to achieve clinical efficacy.
As a leading antibody service provider, Creative Biolabs provides both customized antibody development and antibody functional analysis services based on our advanced technology platforms to help advance your projects. Our professional scientists have extensive experience in antibody functional analysis and are committed to providing high-quality services to promote the development of innovative cancer treatments. Here, we concentrate on target binding-based analysis and characterization.
The variable domain locates at the N-terminal of an antibody that can bind to antigens. It has a set of complementarity determining regions (CDRs) serving as the antigen-binding site. Each arm of the immunoglobulin monomer could bind an epitope on the antigen. For a typical IgG, Fc and Fab fragments can be generated after the cleaving of the papain enzyme. The pepsin enzyme functions below the hinge region, so an F(ab')2 fragment and a pFc' fragment is formed. The variable regions of the heavy and light chains can be combined to form a single-chain variable fragment (scFv), which is only half the size of the Fab fragment, yet maintains the original specificity of the parent immunoglobulin.
The variable domain of antibodies functions by binding to specific antigens of pathogens or different molecules. As a result, one of the major functions is antigen recognition. The binding affinity to soluble antigens or cell membrane receptors greatly decides the efficacy of potential antibody drugs. When an antibody can recognize toxins or pathogens and thereby block the access to host cells and inhibit infections, such function is known as neutralization. Additionally, antibodies can lead to target tumor cell apoptosis by binding to specific receptors. In brief, antibodies can mediate a wide range of biological functions through the binding to their corresponding targets. Therefore, it is essential to evaluate the target binding-based functions of a therapeutic antibody before it is used in clinical trials. Creative Biolabs offers various functional assays to evaluate such characteristics and functions, including but not limited to:
Binding assay is a fundamental process for evaluating antibody-target interactions. Now we provide both ligand binding assays (LBA) and cell-based binding assays (CBA) to assist our clients better understand the binding properties of specific antibody lead, in a most physiologically relevant manner.
Cell proliferation and viability are widely used as an indicator for assessing therapeutic consequences and pharmacological safety. We can offer multiple proliferation assays to detect and evaluate the overall cytotoxic effects of your antibodies.
Antibody-mediated cell apoptosis can directly eliminate target cells by activating various apoptosis signal pathways. Our high-throughput BCL/caspase screening assays can help determine whether the apoptosis mechanism contributes to cell death and exactly which pathway is involved.
Neutralization assay is specially designed to routinely validate and measure antibody neutralization potential against various pathogens, cytokines, toxins, etc. We have tailored various assay formats from which you can select a most suitable one.
Antibody-Receptor Function Studies
The antibody can bind specifically to a receptor in order to exert its intended effects in immunotherapy. Creative Biolabs offers antibody-receptor functional analysis targeting both immune checkpoints and G protein-coupled receptors (GPCRs).
Endocytosis and Internalization Assays
Internalization is a process that antibodies specifically recognize cell surface receptors, thereby inducing receptor-mediated endocytosis. This process is often studied for targeted delivery of drugs, toxins, enzymes or DNA for therapeutic applications. Creative Biolabs has introduced multiple state-of-the-art techniques to in-depth study the receptor/antibody internalizing mechanism during the early stage.
Angiogenesis is a complex process associated with tumor progression and metastasis. Now we can offer angiogenesis investigation services such as the tube formation assay and cell migration assay.
In Vitro Cellular Senescence Assay
Cellular senescence is one of the most fundamental characteristics of cell behavior and functions as a tumor suppressor mechanism and links senescence bypass to the emergence of cancer, which is of great importance for drug development in cancer therapy.
PARP assay is of great significance for the discovery of PARP inhibitors with great therapeutic potential in the treatment of breast and ovarian cancer. Creative Biolabs is committed to providing PARP assay services with the potential to aid in cancer research.
In Situ MicroRNA Detection Service
Analyzing miRNA expression in situ is of great significance in cancer therapy and other pathologies. Creative Biolabs provides a one-stop customized in situ microRNA detection service with long-term practical experience and novel technologies to ensure the ideal results for each customer.
Creative Biolabs can offer comprehensive target binding-based function analysis & characterization services with high-quality and efficiency. Our experienced scientists along with our broad portfolio of services make it easier to meet the demands of different researchers. With years of experience in antibody functional assay, Creative Biolabs has the capability and confidence in tailoring the best-fit assays to meet your specific needs and facilitate your project in time. Please contact us for more information and a detailed quote.
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