TNBS/DNBS-Induced Rodent Colitis Model

Creative Biolabs is one of the most reliable industry leaders specialized in preclinical drug development. For colitis studies and anti-colitis drug development, we provide various chemically-induced rodent models covering TNBS, DSS, OXA, and NSAIDs. Moreover, we offer the most comprehensive services from model selection, model creation to experimental design, data collection, and analysis to guarantee that the most dependable results are obtained.

Overview of IBD Animal Models

Inflammatory bowel disease (IBD) is characterized by chronic relapsing inflammation in the gastrointestinal (GI) tract, resulting in symptoms of diarrhea, weight loss, and abdominal pain. The etiology remains elusive due to multiple factors that act in concert to produce pathologic change. However, the advent of animal models has helped investigators to get a better understanding of the mechanisms involved in IBD. In the last few decades, numerous animal models have been developed to characterize the complexity of IBD pathogenesis, delineating underlying molecular mechanisms and evaluating potential human therapeutics. Animal models in IBD research are used in studies that fall into 5 broad categories: addressing scientific questions, evaluating the preclinical efficacy, evaluating the pharmacokinetics (PK), safety testing, and biomarker studies.

TNBS/DNBS-Induced Colitis Model

Chemical-induced colitis models play a pivotal role in the study of various aspects of IBD, due to their low cost and rapid onset of disease. One such model is that of TNBS/DNBS model, an easily induced and highly reproducible experimental colitis model induced in susceptible strains of mice or rats by employing rectal instillation of the mucosal sensitizing agents DNBS/TNBS, diluted in varying concentrations of ethanol. Ethanol is used as a means to effectively break the intestinal barrier and enable the interaction of TNBS with colon tissue proteins, thereby triggering the host innate and adaptive immune responses.

Fig.1 Immunohistochemical analysis of pSmad1/5/8 in rat colons from TNBS colitis and TNBS/BMP7-treated rats. (Maric et al. 2012)

Features of TNBS/DNBS-Induced Colitis Model

• This model is characterized by bloody diarrhea, loss of body weight and intestinal wall thickening, while symptoms differ depending on the type of rodent used, as well as the timing, dose, and degree of exposure to the DNBS or TNBS used in the study.
• Compared with the DSS model, the advantages of DNBS and TNBS-induced colitis include low cost, rapid development of colitis and consistent localized damage to the distal colon.
• The drawbacks of this model are a requirement for a greater level of technical expertise, optimization of DNBS/TNBS dose, and the need for anesthesia for rectal administration.
• Compared to DNBS, TNBS is considered as a preferred choice of chemical to induce colitis due to its highly oxidative properties.

Assessments

Creative Biolabs provides all kinds of assessments for testing the effectiveness of potential drugs depending on your research objectives, including but not limited to:

• Body weight
• Clinical score
• Stool consistency and hemoccult positivity
• DIA (Disease Activity Index)
• Colon length & weight
• Histology & immunohistochemistry
• Intestinal bleeding and permeability
• Epithelial cell proliferation and migration
• Myeloperoxidase (MPO) assay
• Endoscopy

Meanwhile, Creative Biolabs also offers other types of rodent digestive system disease models that you might be interested in. They are listed as follows for you to review:

• Ethanol-Induced Rodent Gastric Ulcer Model
• Carbon Tetrachloride (CCl₄)-Induced Rodent Hepatic Injury Model
• Dextran Sulfate Sodium (DSS)-Induced Rodent Colitis Model
• Indomethacin-Induced Rodent Inflammation in Small Intestine
• Oxazolone (OXA)-Induced Rodent Colitis Model
• AVV-HBV-Induced Chronic HBV Infection Rodent Model
• Rodent Woodchuck Model of Chronic Hepatitis B

At Creative Biolabs, a wide array of well-characterized IBD models and a variety of administration routes and clinically relevant endpoints can be tailored to your digestive discovery projects. Please don't hesitate to contact us if you are interested in any of these services.

Reference

1. Maric, I.; et al. BMP signaling in rats with TNBS-induced colitis following BMP7 therapy[J]. American Journal of Physiology Gastrointestinal & Liver Physiology. 2012, 302(10): G1151.

For Research Use Only.