Creative Biolabs has brought a full range of in vitro cell-based assays in an effort to help our clients better understand the characteristics and mechanism of action of their therapeutic antibody candidates. Particularly, we now offer professional service of virus neutralization assays for studying the potency or blocking the activity of anti-virus antibodies.
Monoclonal antibodies (mAbs) are increasingly being considered as agents to fight severe viral diseases. They are selected and used based on their virus-neutralizing activity and/or cell-killing activity to blunt viral propagation via direct mechanisms. So far, most antiviral mAbs with therapeutic potential have initially been selected for their ability to neutralize virions via the recognition of viral surface antigens essential for receptor binding and/or entry into host cells. However, besides direct targeting of viruses, these antibodies can also trigger effector functions mediated by their Fc fragment, eliciting complement-dependent cytotoxicity (CDC), antibody dependent cellular phagocytosis (ADCP), and antibody-dependent cell-mediated cytotoxicity (ADCC) to eliminate infected cells displaying viral antigens at their surface. These characteristics have demonstrated that antiviral antibodies represent promising, high-added-value therapeutic agents.
Due to the importance of virus neutralizing activity for antiviral antibodies, it is necessary to evaluate the potential of antibody candidates to neutralize the target viruses. Virus neutralization assay is an in vitro test to detect the potency of antibodies to block virus infectivity. This assay has been widely used to target a number of viruses, such as H5N1 influenza virus, human immunodeficiency virus (HIV), herpes simplex virus (HSV), cytomegalovirus (CMV), hepatitis C virus (HCV), Ebola virus, and so on.
Fig. 1 Pseudotype-based viral neutralization assay.1
The virus neutralization assay is performed through co-incubation of the virus, antiviral antibodies, and target cells. Infectivity is identified by the presence of a cytopathic effect on target cells, or alternatively reduction of proliferative effects. The plaque reduction neutralization test (PRNT) is a gold standard method to measure the neutralization potency of antiviral antibodies. For test materials, we can deal with both purified antibodies and rough samples. Moreover, we offer a full menu of pathogenic viruses and pseudotyped viruses that are available to help you find the most suitable target. Appropriate controls are applied to ensure assay validity.
Our exhaustive service of antibody functional assays can guarantee our clients with the most compelling and reproducible outcomes, offering absolute confidence for subsequent investigations. Additionally, Creative Biolabs has built a team of experienced scientists with facilities and processes designed specifically to provide the best strategy and protocols customized to antibody functional characterization services. For more detailed information, please feel free to contact us or directly sent us an inquiry.
Reference
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