Fetal bovine serum, FBS for short, is derived from bovine serum which contains high levels of bovine exosome vesicles. It is an essential component of many types of cell media. Isolated exosomes from cultured cells may contain bovine exosomes because of the presence of FBS in cell media, which leads to some interference factors in the study of exosome function, even misleading experimental results. In order to solve this problem, Creative Biolabs has developed exosome-depleted FBS. This product supports the equivalent growth of a variety of cell type in the culture. Moreover, experimental date revealed that the very low levels of bovine CD63 positive exosomes and no measurable bovine microRNAs are detected in exosome-depleted FBS media supplement.