Exosome Isolation Services

With an unwavering commitment to excellence, Creative Biolabs offers you the opportunity to access exosomes from a wide range of biological samples, empowering your research endeavors with versatility and precision. Whether you're pursuing biomarker discovery, functional studies, or comparative analysis, our comprehensive services ensure that you have the right exosome samples to fuel your research goals.

Exosome Isolation Services from Multiple Sources

Table 1 Summary of various types of exosomes that can be isolated.

Specific Service Items Description Application Prospects
Animal Cell-derived Exosome Isolation Exosomes derived from animal cells are tiny cell secretions. Almost all animals can release exosomes, such as stem cells, B cells, dendritic cells and mast cells, and tumor cells. These animal cell-derived exosomes play important roles in intercellular communication, immune response, developmental regulation, and disease progression. Because the biomolecules carried by animal cell-derived exosomes can transmit information between cells, researchers are increasingly studying animal cell exosomes, hoping to use them to develop new treatments, diagnostic tools, and biomarkers.
Body Fluid-derived Exosome Isolation Exosomes sourced from bodily fluids typically refer to vesicles present in the liquid environments within organisms, such as plasma, serum, urine, cerebrospinal fluid, and interstitial fluid. Research into exosomes derived from these bodily fluid sources is continuously advancing, as scientists explore their roles in physiological and pathological processes, as well as how to leverage them for the development of novel diagnostic, therapeutic, and monitoring approaches.
Plant-derived Exosome Isolation Plant-derived exosomes are a type of cell-secreted substance similar to animal cell-derived exosomes. It has been discovered that exosomes from various plant sources hold potential applications in disease treatment, health care, and skin improvement. Scientists have begun to gradually unveil their functionalities and potential uses.
Microorganism-derived Exosome Isolation Microorganisms can also release exosomes through fusion or secretion pathways. Microorganism-derived exosomes play a significant role in microbial communication, pathogenicity, and symbiotic relationships. Researchers are actively exploring the functionalities of microorganism-derived exosomes, including their roles in disease development and host immune responses. These studies offer potential opportunities for developing new medical and biotechnological applications.

Methods Overview for Exosome Isolation

Table 2 Summary of various methods for exosome isolation.

Methods Principle Schematic Diagram Advantages Disadvantages
Ultracentrifugation Exosomes are separated based on their density using high-speed centrifugation. Schematic of differential ultracentrifugation–based exosome isolation. (Liu, et al., 2022)
Fig.1 Schematic of differential ultracentrifugation–based exosome isolation.1
Established and widely used method can purify exosomes from different biological fluids. Labor-intensive, time-consuming, may co-precipitate non-exosome materials, potential damage to exosomes due to high forces.
Density Gradient Centrifugation Exosomes are separated based on their buoyant density by gradient centrifugation. Schematic of gradient density ultracentrifugation-based exosome isolation. (Liu, et al., 2022)
Fig.2 Schematic of gradient density ultracentrifugation-based exosome isolation.1
Good purity. Complex, requires careful gradient preparation, longer procedure.
Ultrafiltration Exosomes are filtered through a membrane with a specific pore size to separate them from larger particles. Schematic of ultrafiltration-based exosome isolation. (Liu, et al., 2022)
Fig.3 Schematic of ultrafiltration-based exosome isolation.1
Simple and relatively quick, suitable for large-volume samples. This may result in co-isolation of proteins and contaminants.
Size-Exclusion Chromatography (SEC) Exosomes are separated based on size by passing through a porous column. Schematic of size-exclusion chromatography-based exosome isolation. (Yang, et al., 2020)
Fig.4 Schematic of size-exclusion chromatography-based exosome isolation.2
The gentle method that preserves exosome integrity. Possible co-elute with proteins, which requires specialized equipment.
Precipitation Polymers are added to induce exosome aggregation and precipitation. Schematic of polymer precipitation–based exosome isolation. (Liu, et al., 2022)
Fig.5 Schematic of polymer precipitation–based exosome isolation.1
Easy and rapid process, good yield from various biological fluids. Contamination with polymers, potential disruption of exosome integrity.
Affinity-based Capture Antibodies are used to capture exosomes based on specific surface markers. Schematic of immunoaffinity-based exosome isolation. (Yang, et al., 2020)
Fig.6 Schematic of immunoaffinity-based exosome isolation.2
High specificity, enables targeted isolation of exosome subpopulations. Limited by the availability of well-characterized antibodies, potential loss of non-targeted exosomes.
Microfluidics-based Isolation Microfluidic devices sort and isolate exosomes based on size, surface markers, or acoustic properties. Integrated microfluidic technique allows combined exosome isolation and analysis. (Yang, et al., 2020)
Fig.7 Integrated microfluidic technique allows combined exosome isolation and analysis.2
Precise control, potential for automation, suited for rare exosome subpopulations. Complex setup may require technical expertise.

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Embark on a journey of discovery by partnering with Creative Biolabs. Contact us today and explore the possibilities that exosome isolation services from multiple sources can bring to your research.

References

  1. Liu, WZ.; Ma, ZJ.; et al. Current status and outlook of advances in exosome isolation. Analytical and Bioanalytical Chemistry. 2022. 414(24):7123-7141.
  2. Yang, D.; Zhang, W.; et al. Progress, opportunity, and perspective on exosome isolation-efforts for efficient exosome-based theranostics. Theranostics. 2020. 10(8):3684-3707.
For Research Use Only. Cannot be used by patients.
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