Four-Dimensional Exosomal Proteomic Detection Service

The basic principle of mass spectrometry (MS) proteomics is to determine the physicochemical properties of the tested sample ions so that qualitative and quantitative results can be obtained based on the sample mass spectra and related information. 4D-Proteomics is the addition of a fourth dimension, ion mobility, to the three dimensions of retention time, m/z, and ion intensity, thereby significantly increasing peak volume, scan speed, and detection sensitivity. This leads to an overall enhancement of proteomics performance in terms of identification depth, detection cycle time, and quantitative accuracy. Creative Biolabs provides proteomics services for 4D exosomes to facilitate proteomics and high-throughput modifications in micro samples and large sample groups.

Introduction of Four-Dimensional Exosomal Proteomic

In recent years, the technique of separation in the GC based on ion size and structure has become the focus of attention in the field of MS. This technique has not only ignited public interest in ion mobility in terms of efficiency and convenience but also because of its ability to combine the advantages of traditional liquid chromatography (LC) and MS techniques. Ion mobility spectrometry (IMS) is the separation of ions in the GC based on size and form by additional one-dimensional ion mobility. Ion mobility has been first introduced to large-scale proteomics analysis, bringing proteomics into a new era of 4D.

Traditional proteomics MS is generally based on the three dimensions of retention time, m/z, and ion intensity to identify and quantify proteins, i.e. 3D proteomics. 4D proteomics adds a fourth dimension, ion mobility, to 3D proteomics, mainly based on the shape and section of ions to be separated, enabling the differentiation and identification of low-abundance protein signals. 4D Proteomics is based on the timsTOF Pro mass spectrometer, which combines TIMS (trapped ion mobility spectrometry) and other technologies to measure the collision cross-section of all detected ions repeatedly, enabling faster and more sensitive proteomic characterization and quantification.

Fig.1 Online parallel accumulation - serial fragmentation (PASEF) with the timsTOF Pro. (Meier, 2018)

Sample requirements at Creative Biolabs

At Creative Biolabs, we offer 4D proteomics assays for exosomes from many types of sample sources, such as Co-IP samples, tissue samples, and protein samples.

Co-IP samples have a protein requirement of around 2-5ug, no detergent (e.g. SDS), and a high oxygen concentration. Or the beads with the protein samples can be sent to us directly, and then we will undertake the subsequent experiments.
If tissue samples are provided, please send the tissue samples in dry ice conditions.

Tissue sample requirements

Protein samples with a total protein of 50ug or more. For protein extraction, ordinary tissue, and cell lysis solution can be used.
Sample transportation: use a sufficient amount of dry ice for transportation and ship fresh samples as timely as possible to reduce the possibility of sample degradation during transportation.

A detailed report on the 4D Exosomal Proteomic service will be provided at Creative Biolabs, which includes

The experimental procedures.
Relevant experimental parameters.
MS images.
Raw data.
Proteomics analysis results.

Currently, the human proteome project includes MS data covering approximately 90% of human proteins, with mapping to other species' proteins. However, the depth of proteomic identification of complex systems is still limited by the liquid phase separation capability, MS scan speed, and sensitivity. Creative Biolabs provides a 4D exosome proteomics service, which optimizes the detection cycle time, depth of identification, and high throughput performance based on traditional proteomic identification to facilitate the in-depth proteomics of exosome-related complex samples. Please feel free to contact us.

Reference

Meier, F.; et al. Online parallel accumulation-serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer. Mol Cell Proteomics. 2018, 17(12): 2534-2545.

Plant Exosome Isolation