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Custom Degenerated Oligo Synthesis


Degenerate oligonucleotide sequences (DOS) and PCR have been successfully used to clone cDNAs of specific proteins. Creative Biolabs relies on the reverse translation of protein subsequences to synthesize limited degenerate oligonucleotides. These products can be used as probes for Northern and Southern blot, as well as cDNA screening and genomic recombinant DNA libraries. DOS primers have also been used to amplify unknown members and homologous genes of gene families in different species.

Applications of Degenerated Oligo

Genetic analysis of a limited amount of genomic DNA can play an important role in research in the fields of justice, paleontology, genetic disease diagnosis, genetic linkage analysis, and genetic diversity. To meet our customers' scientific needs, we organically synthesize one or two purine nucleoside analogs to incorporate them into the oligonucleotide. The particular structures will be chosen to give them the character of hydrogen-bond ambiguity (or degenerate). Degenerate oligonucleotides can amplify human genomic DNA by initiating degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), increasing the number of templates for microsatellite repeat labeling genotyping. Especially for smaller genomes, DOP-PCR can amplify 200-600 times.

In summary, DOP-PCR-amplified genomic DNA is an excellent and reliable template for microsatellite genotyping and can produce noticeable bands. In all the discrete microsatellites tested, the amplification of all genomic DNA was equal, which means that the human genome was almost completely covered. Therefore, DOP-PCR seems to allow unbiased, hundreds-fold, whole-genome amplification of human genomic DNA for genotyping.

Amplification of cDNA using degenerate oligonucleotide sequence primers. Figure 1. Amplification of cDNA using degenerate oligonucleotide sequence primers. (Cooper, 1991)

Custom Degenerated Oligo Synthesis

Creative Biolabs customizes the synthesis of degenerated oligonucleotides by using the following methods:

Features

If you have any questions about our custom degenerated oligo synthesis service, you can contact us by email or send us an inquiry to find a complete solution.

Reference

  1. Cooper, D.L.; Baptist, E.W. (1991). Degenerate oligonucleotide sequence-directed cross-species PCR cloning of the BCP 54/ALDH 3 cDNA: priming from inverted repeats and formation of tandem primer arrays. Genome Research. 1(1): 57-62.

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