Custom miRNA Inhibitor Synthesis Service
Introduction
Creative Biolabs' Custom miRNA Inhibitor Synthesis service accelerates target validation and therapeutic development via advanced oligonucleotide chemistry and strict QC. Precisely engineered to block target miRNAs, our inhibitors enable accurate gene silencing for biological research and drug development, overcoming non-specific modulation to clarify experimental outcomes and streamline timelines.
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Custom miRNA Inhibitor Synthesis
MicroRNAs (miRNAs) are critical post-transcriptional gene regulators, small non-coding RNAs that fine-tune gene expression by binding to mRNAs, inducing degradation or translational repression. Dysregulated miRNA activity is implicated in diverse human pathologies, including cancer, cardiovascular diseases, metabolic disorders, and viral infections. Custom miRNA inhibitors (antagomirs/anti-miRs) are synthetic oligonucleotides designed to specifically sequester mature miRNAs via stable duplex formation, preventing mRNA binding and thereby de-repressing target gene expression. This precise modulation enables functional elucidation of miRNAs in biological processes and therapeutic exploration of aberrant miRNA-driven diseases.
Fig.1 Various mechanisms by which small molecule inhibitors target and inhibit specific miRNA.1
Workflow
Synthesis
The production of custom miRNA inhibitors involves sophisticated chemical synthesis techniques. At Creative Biolabs, we employ state-of-the-art automated oligonucleotide synthesizers to build the inhibitor sequence nucleotide by nucleotide. A critical aspect of this process is the incorporation of various chemical modifications that are essential for enhancing the inhibitor's performance in vitro and in vivo.
| Target Information | Modification | Purification and QC | Quantities |
|---|---|---|---|
| Identify sense sequence (5'→3') |
2'-O-Methyl (2'-OMe) and 2'-O-Methoxyethyl (2'-MOE) Enhance nuclease resistance, improve affinity with target miRNA, and avoid degradation. |
Analytical HPLC Provides a final assessment of the purity of the oligonucleotide, quantifying the percentage of the full-length product and identifying any remaining impurities. |
Identify required quantities, such as 50 nmol, 100 nmol, or 200 nmol. |
| Identify antisense sequence (Optional, 5'→3') |
Locked Nucleic Acid (LNA) The thermal stability and binding affinity to the target miRNA were improved, and the specificity was also improved. |
Mass Spectrometry (MS) Used to precisely determine the molecular weight of the synthesized oligonucleotide, confirming the correct sequence and incorporation of all specified chemical modifications. |
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Phosphorothioate (PS) Backbone Increases resistance to nuclease degradation and prolongs the half-life of the inhibitor in vivo. |
Polyacrylamide Gel Electrophoresis (PAGE) Performed under denaturing conditions to assess the integrity of the oligonucleotide, ensuring the absence of significant degradation products or truncated sequences. |
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| Click for a full list of available modifications | |||
| Final Deliverables |
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| Estimated Timeframe | The typical timeframe for custom miRNA inhibitor synthesis projects ranges from 4 to 8 weeks, depending on the complexity of the desired chemical modifications, the scale of synthesis, and the overall scope of the project. | ||
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What We Can Offer
Our custom miRNA inhibitor synthesis service is meticulously designed to empower your most ambitious biological research and therapeutic development. We don't just synthesize; we partner with you to deliver precision tools that drive discovery.
Tailored Design & Optimization
Fully customized miRNA inhibitor design with sequence and chemical modification optimization for target-specific potency and specificity.
Advanced Synthesis Capabilities
State-of-the-art automated platforms for research-to-preclinical scale synthesis, adapting to project needs.
Stringent Purity & QC
HPLC-based multi-stage purification with Mass Spectrometry, PAGE, and Analytical HPLC validation for batch reproducibility.
Stability & In Vivo Enhancement
Strategic chemical modifications for nuclease resistance and prolonged half-life in complex biological systems.
Expert Consultation
Specialist guidance from design to delivery strategy, including EV-based formulations.
Accelerated R&D
High-specificity inhibitors to streamline target validation and miRNA-targeted therapeutic development.
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Customer Reviews
FAQs
What is the maximum length of the miRNA sequence that can be synthesized for custom miRNA inhibitors?
Our synthesis capabilities generally allow for the synthesis of miRNA inhibitors with sequences ranging from 18 to 30 nucleotides in length. If you have requirements for longer sequences, we will assess the feasibility and discuss the solutions with you.
Can custom miRNA inhibitors be synthesized with fluorescent labels?
Yes, we offer fluorescent labeling options for custom miRNA inhibitors. Fluorescently labeled inhibitors can be used for tracking and visualization in various experiments. You can inform us of your specific labeling requirements, and our team will help you select the most suitable labeling method.
How to validate the effectiveness of custom miRNA inhibitors?
We usually validate the effectiveness of miRNA inhibitors through cellular and animal experiments. By observing the changes in miRNA function and related biological effects in cells or animal models, we can determine whether the inhibitor can specifically bind to the target miRNA and play a regulatory role.
Can custom miRNA inhibitors be synthesized with modified backbone structures?
Yes, we can synthesize custom miRNA inhibitors with modified backbone structures, such as phosphorothioate (PS) backbone modifications. These modifications can enhance the stability of inhibitors, making them more resistant to nuclease degradation.
Creative Biolabs is your trusted partner for Custom miRNA Inhibitor Synthesis, providing high-purity, precisely engineered oligonucleotides designed to meet the rigorous demands of modern biological research and therapeutic development. From initial design consultation to comprehensive quality control, we are dedicated to empowering your breakthroughs in gene modulation.
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Reference
- Seyhan, Attila A. "Trials and tribulations of MicroRNA therapeutics." International journal of molecular sciences 25.3 (2024): 1469. DOI: 10.3390/ijms25031469. Distributed under Open Access license CC BY 4.0, without modification.
