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Custom shRNA Lentivirus Library


Vector-based shRNA (short-hairpin RNA) expression library has been widely used to screen functional genes. Especially, lentivirus is widely used as a vehicle to deliver shRNAs to target cells because it can provide long-term gene silencing with high delivery efficiency. As a leading company in the field of gene therapy, Creative Biolabs is capable of offering custom shRNA lentivirus library targeting multiple genes.

shRNA Library for RNA Interference (RNAi)

Over the past years, RNAi screens have become a central method in the field of functional genomics to identify critical cancer pathways, molecular drug targets, and therapeutic synergies. With the construction of shRNA libraries, large-scale screens can now be efficiently performed without expensive liquid- and plate-handling automation. In the pooled shRNA library, thousands of different shRNAs are introduced into a cell population, which is then selected for a phenotype of interest. Cells expressing shRNAs that target genes involved in this phenotype are either depleted or enriched compared to a non-selected control population. Candidate genes that are potentially targeted by the enriched or depleted shRNAs can be further investigated by downstream functional studies.

 shRNA library screening of Abl kinase-induced cell detachment. Figure 1. shRNA library screening of Abl kinase-induced cell detachment. (Huang, 2008)

Custom shRNA Lentivirus Library Services at Creative Biolabs

shRNA library preparation is a multi-step process, and each step can affect the quality of data and resulting screen hits. With extensive experience in library construction, Creative Biolabs is able to produce completely new, high-quality custom libraries focusing on any target set of genes using our optimized lentivirus vector for large-scale functional screens. We can deliver your library in both plasmid and pre-packaged ready-to-transduce formats, depending on your requirements. Our professional scientists will help you choose the optimal promoter and fluorescent reporter to satisfy your in vitro or in vivo screening. Additionally, we can even achieve titers up to 109 TU/mL.

Advantages of Our Services

Creative Biolabs is committed to accelerating the development of gene therapy programs. If you are interested in our custom shRNA lentivirus library service, please feel free to contact us and tell us what gene you want to target. Our scientists will send you a service proposal including price, turnaround and other relevant information as soon as possible.

Reference

  1. Huang, X.; et al. (2008). Systems analysis of quantitative shRNA-library screens identifies regulators of cell adhesion. BMC systems biology. 2(1), p.49.

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