Small hairpin RNA (shRNA) has been used as a research tool to control the expression of specific genes in numerous experimental organisms and to promote the development of gene therapeutics methods. As a tool to deliver genetic material into cells (in vivo or in vitro), lentiviral vectors are widely used to integrate shRNA into the genome of both dividing and non-dividing cells and thus down-regulate the specific gene. As a leading company providing comprehensive gene therapy solutions, Creative Biolabs has developed a top technology platform to offer custom shRNA lentivirus service for any specific gene.

Procedures of Our Custom shRNA Lentivirus Service

Generally, four main procedures are included to construct shRNA lentiviral vectors, namely (1) target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, (3) using packaging cells to produce shRNA lentivirus, and (4) transducing target cells with shRNA lentivirus. With extensive experience in lentivirus vectors construction, Creative Biolabs could produce high-quality ready-to-use shRNA lentivirus based on the knockdown gene information (sequence or Gene ID) appointed by the customers. Besides, we can help you choose small interfering RNA (siRNA) sequences within the target gene if you can’t already provide an efficient knockdown sequence. Moreover, we will design a set of shRNA constructs in which one of them will show a knockdown of ≥70% of the target RNA.

Figure 1. Delivery of shRNA by lentiviral vectors. (Brea, 2017)

Features of Our Services

• Professional scientists with years of experience in lentiviral cloning and expression;
• Flexible forms of cooperation which are suitable for both specific gene sequence and gene ID;
• Recombinant shRNA lentivirus vectors can transfect a wide range of cell lines including non-dividing, primary or stem cells;
• Multiple delivery formats (3 individual constructs of 5 µg purified plasmid and a separate scrambled control plasmid) with high titer (≥ 1 x 10⁷ Transducing Units/mL) and fluorescent protein marker (mCherry or eGFP reporter genes);
• Fast turnaround time at a relatively low price.

Creative Biolabs has been devoted to offering ready-to-use shRNA lentivirus to promote the development of our global customers’ programs. Normally, we provide a set of three expression constructs and a scrambled control for every target gene. Our experienced scientists will do all they can do to deliver safer and higher titer shRNA lentivirus vectors for both in vivo and in vitro research. Please feel free to contact us for more details and our scientists will have a further in-depth discussion on your project.

Reference

1. Brea, M.S.; et al. (2017). Intramyocardial gene silencing by interfering RNA.

For Lab Research Use Only, Not for Human or Animal Therapeutic Use.