Custom Viral Vector Production Services
Introduction
Gene therapy, a revolutionary approach modifying genes to treat diseases, hinges on safe, efficient delivery systems. Creative Biolabs' Custom Viral Vector Production Services address critical challenges like vector design and regulatory compliance. We engineer high-titer, scalable viral vectors (Lentivirus, Adenovirus, AAV, HSV, Vaccinia) for precise gene transfer, offering tailored solutions with expert support to accelerate R&D and clinical success.
Viral Vector
Distributed under CC BY 4.0, from wiki, without modification.
| Viral Vector | Structural and Genomic Features | Typical application scenarios | Titers | Capacity of insertion | Immunogenicity | Characteristics of expression |
|---|---|---|---|---|---|---|
| Lentiviral Vector | Enveloped virus, 80-100 nm in diameter, single-stranded RNA genome (7-12 kb) containing helper genes | Nervous system disease research, hematopoietic stem cell gene therapy, CAR-T cell engineering, and chronic infectious disease research | 10⁷-10⁹ IU/mL | ~8 kb | Lower | Long-term stable expression |
| Adenovirus Vector | Unenveloped, icosahedral, 70-100 nm in diameter, double-stranded DNA genome (26-40 kb), often missing E1 and E3 regions | Tumor gene therapy, liver disease treatment, and vaccine development | 10⁹-10¹¹ IU/mL | The first generation is about 7.5 kb, and the "gutted" vector is 36 kb | High | Transient expression |
| Adeno-associated Viral Vector | Non-enveloped, icosahedral, approximately 22 nm in diameter, single-stranded DNA genome (approximately 4.7 kb) with ITR sequences at both ends | Treatment of monogenic genetic diseases, hereditary retinal diseases, and neurological diseases | 10⁷-10⁹ IU/mL | ~4.7 kb | Low | Long-term expression |
| Herpes Simplex Virus Vector | Enveloped virus with icosahedral capsid, double-stranded DNA genome (~152 kb) | Brain tumor treatment, neurodegenerative disease treatment | - | Up to 150 kb | Low (replication-deficient) | The incubation period can be established |
| Vaccinia Virus Vector | Complex, brick-shaped enveloped viruses, with double-stranded DNA genomes (e.g., MVA 178 kb), replicate in the cytoplasm. | Virus/bacterial/parasite vaccine development, tumor immunotherapy | - | Large amounts of foreign DNA can be inserted | High | Transient expression |
Workflow
| Viral Vector | Synthesis Technology | Purification Technical |
|---|---|---|
| Lentiviral Vector |
1. Vector construction Three or four plasmid systems 2. Packaging system 293T cell transient transfection: calcium phosphate method or liposome transfection |
Cell debris is removed by low-speed centrifugation, followed by ultra-high-speed centrifugation to precipitate virus particles. The virus is then purified via affinity/ion-exchange chromatography and concentrated by ultrafiltration, centrifugation, or PEG precipitation. |
| Adenovirus Vector |
1. Vector construction
293 cells were serially passaged and expanded: multiplicity of infection (MOI) 10-100, cultured for 3-5 days until cytopathic effect. |
1. The classical approach CsCl density gradient centrifugation 2. Column chromatography
|
| Adeno-associated Viral Vector |
1. Vector construction
|
1. Two-step purification method
Column chromatography combined with ultrafiltration |
| Herpes Simplex Virus Vector |
1. Vector construction
Infection of Vero cells or primary nerve cells |
1. Centrifugation and purification 2. Chromatography Sucrose density gradient centrifugation (20%-60% sucrose) is used to isolate virus particles 3. Purification optimization Proteinase K treatment removed host DNA/protein, and DNase I digested free nucleic acids |
| Vaccinia Virus Vector |
1. Vector construction
BHK-21 or HeLa cells were infected |
1. Classical purification After repeated freezing and thawing, the cells are lysed by sonication and differential centrifugation 2. Density gradient centrifugation Sucrose (30%-60%) or Percoll gradient centrifugation |
What We Can Offer
As a leader in custom viral vector production, Creative Biolabs offers unparalleled capabilities designed to meet the rigorous demands of your gene therapy and biopharmaceutical projects. Our commitment to precision, scalability, and quality ensures optimal outcomes.
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- Comprehensive Vector Design & Optimization
Tailored genetic design and optimization of Lentivirus, Adenovirus, AAV, HSV, and Vaccinia vectors for target cell tropism and expression requirements.
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- Scalable Production Capabilities
Robust platforms for research-to-clinical grade viral vector production, ensuring consistent high-titer batches.
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- Advanced Purification Technologies
State-of-the-art TFF and chromatography (affinity/ion-exchange) for high-purity vectors, minimizing empty capsids and contaminants.
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- Rigorous Quality Control & Analytical Services
QbD-driven QC system with titer/purity/identity testing and functional potency assessment.
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- Customization for Complex Projects
Bespoke solutions for multi-gene constructs, serotype modifications, and complex experimental designs.
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- Dedicated Regulatory Support
Stringent compliance with detailed documentation to facilitate regulatory submissions.
Customer Reviews
FAQs
Q: What factors primarily influence the choice of viral vector for my specific research or therapeutic application?
A: The optimal viral vector depends on several key factors, including your target cell type (dividing vs. non-dividing), desired gene expression duration (transient vs. long-term), the size of your gene of interest, the required viral titer, and critical safety considerations like immunogenicity and integration risk.
Q: How does Creative Biolabs ensure the quality, purity, and safety of its custom viral vectors?
A: At Creative Biolabs, quality is our top priority. We use optimized production methods, advanced purification techniques like chromatography and tangential flow filtration, and strict QC testing. Our tests check titer, purity, sterility, identity, aggregation, and potency, meeting industry standards to ensure each batch meets your needs and safety requirements.
Q: Can I request a custom vector design that is not explicitly listed in your standard offerings or requires unique modifications?
A: "Custom" is at the core of our services. We specialize in tailoring viral vector designs to precise client specifications. Whether you require specific promoter-enhancer elements, unique tropism modifications, multi-gene constructs, or advanced safety features, our expert scientists work collaboratively with you to design and produce vectors that perfectly align with your innovative research goals.
Q: What are the key differences between Lentiviral Vectors and AAV Vectors for gene delivery?
A: Both LV and AAV can achieve long-term gene expression in nondividing cells, but there are essential differences between the two. LVS can be integrated into the genome for stable expression and can carry up to 8kb in length, but the risk of insertion mutation is low. AAV remained free of expression, provided good safety and tissue-specific serotypes, but carried only 4.7 kb.
Contact Our Team for More Information and to Discuss Your Project
