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DNA/2'-O-Methyl RNA Chimera

The incorporation of 2'-O-Methyl RNA bases in antisense oligonucleotides or miRNA can increase the stability of these oligos and prevent them from degradation by nuclease, as well as improve their affinity to the target mRNA by increasing the Tm. With years of experience in custom oligonucleotide modifications, Creative Biolabs offers a full range of oligonucleotide modification services including 2'-O-Methyl modification. Our scientists are proficient in the preparation of DNA/2'-O-Methyl RNA chimera with high purity and high quality to promote clients’ meaningful research.

Introduction of 2'-O-Methyl RNA

2'-O-methylation is a common nucleoside modification of RNA, in which a methyl group is added to the 2' hydroxyl of the ribose moiety of a nucleoside to produce a methoxy group. 2'-O-Methyl RNA is a naturally occurring modification of RNA found in tRNA and small RNAs. It represents one of the earliest sugar modifications employed in antisense medicinal chemistry and is widely used to improve the nuclease stability of siRNA or antisense oligonucleotides. A number of studies have revealed that the oligonucleotides modified with 2'-O-Methyl RNA showed higher nuclease resistance and affinity to target mRNA compared to unmodified oligonucleotides. Especially, the combination of 2'-O-Methyl RNA with phosphorothioate linkages is recommended to be introduced in the process of oligos synthesis in order to further increase the nuclease resistance.

2'-O-methyl-adenosine, a modified adenosine. Figure 1. 2'-O-methyl-adenosine, a modified adenosine.

Our Services

Equipped with rich expertise in oligonucleotide production and chemical modifications, Creative Biolabs is confident in offering unbeatable synthesis services of RNA and DNA chimeric oligos with 2'-O-Methyl modification for global clients. Our advanced oligonucleotide synthesis platform is composed of nucleic acid synthesizers, nucleic acid purification systems, and nucleic acid analysis instruments, allowing delivery of micrograms or even hundreds of grams oligos in a short time. Besides, we also provide a variety of strategies to improve properties of chimeric oligos, for example, by adding 2'-O-Methyl RNA and phosphorothioate linkages into oligos to further improve their nuclease resistance, by retaining an RNase H activating domain in 2'-O-Methyl RNA chimeric oligos to enable them possess the activity of RNase H cleavage.


Creative Biolabs has organized an expert team with extensive experience in the field of custom oligo synthesis and modification. We are committed to providing end-to-end custom oligo modification services from consultation, oligo design, production, and purification, to oligo quality control and delivery. Please don’t hesitate to contact us for more details.

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