Lipid Nanoparticle (LNP) Conjugation Solution
Creative Biolabs leverages its deep expertise in precision microfluidics, advanced bioorthogonal chemistry, and rigorous multi-level quality control to create an industry-leading platform. We are committed to systematically overcoming the core challenges of drug development—the safe, efficient, and precise delivery of complex therapeutic payloads to lesion sites.
From initial design and feasibility studies to large-scale manufacturing and comprehensive final characterization, our collaborative processes ensure scientific excellence and accelerated translational applications.
Our Services
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Custom ligand-LNP conjugation: Covalently linking virtually any bioactive molecule (peptide, antibody, aptamer, carbohydrate, small molecule) to the LNP surface.
- Antibody-LNP conjugate development
- Multifunctional LNP engineering
- Radiolabeling and imaging LNP functionalization
- Comprehensive analytical and bioconjugate characterization
Our Collaborative Process
Our collaboration model with clients is transparent, iterative, and scientifically rigorous, ensuring a seamless transition from concept to preclinical trials.
Consultation & Target Definition
Our PhD-level scientists will work with you to identify target cells, payload, required pharmacokinetics, and the final application.
Design & Protocol
We will propose detailed conjugation strategies, including lipid composition selection, linker chemistry, and functional group recommendations, and provide preliminary data support where feasible.
LNP Formulation & Conjugation
Our team handles the synthesis and employs rigorous quality control to ensure batch-to-batch consistency.
In Vitro/In Vivo Validation
We will provide comprehensive data on conjugation efficiency, binding affinity (e.g., surface plasmon resonance), cellular uptake by target and non-target cells, and in vivo biodistribution.
Delivery & Reporting
You will receive a fully characterized conjugated LNP, a comprehensive report, and ongoing scientific support from us.
Why Need LNP Conjugation?
The necessity for LNP conjugation is driven by the limitations of untargeted LNPs, which predominantly accumulate in the liver. Conjugation enables precision medicine at the nanoscale.
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CNS Disorders
Conjugation of ligands targeting the transferrin receptor (TfR) or insulin receptor has been shown to significantly enhance LNP uptake into the brain.
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Vaccines
Targeting LNPs to specific immune cells by conjugating antibodies can dramatically enhance the potency and durability of immune responses for both infectious disease and cancer vaccines.
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Gene Therapy & Editing
Targeted LNPs can minimize this risk by delivering the gene-editing machinery exclusively to the intended cell type, enhancing both efficacy and safety.
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Diagnostic Imaging
Conjugating radionuclides or fluorescent dyes allows for non-invasive tracking of LNP biodistribution, a crucial tool for preclinical development and potentially for clinical diagnostics.
Find the Right Lipid Nanoparticle for Your Conjugation Research
Ionizable Lipids
- • Description: Ionizable lipids play a crucial role in promoting the efficient encapsulation of nucleic acids in lipid nanoparticles (LNPs), typically achieving encapsulation efficiencies exceeding 90%.
- • Applications: These lipids are essential for the successful development of LNPs due to their multifaceted functions.
Pegylated Lipids
- • Description: Pegylated lipids play multiple functions in LNP formulations and are essential for mRNA delivery. In lipid nanoparticle (LNP) formulations, the lipid content determines their key properties.
- • Applications: Furthermore, pegylated lipids help enhance the stability of LNPs and reduce their aggregation. They also protect LNPs from uptake by the mononuclear phagocyte system (MPS).
Phospholipids
- • Description: Phospholipids not only provide structural stability to nanoparticles but, depending on their type, can also induce lipid bilayer disruption, promoting endosome escape.
- • Types: One commonly used phospholipid is phosphatidylcholine (PC), including its variants such as 1,2-distearate-sn-glycerol-3-phosphate choline (DSPC) and hydrogenated soybean phosphatidylcholine (HSPC).
Cholesterol
- • Description: Cholesterol plays a crucial role as a key component of lipid nanoparticles (LNPs), enhancing particle stability by regulating membrane integrity and rigidity.
- • Applications: In LNP formulations, cholesterol is typically added in an equimolar amount to the endogenous membrane.
Lipid Nanoparticle (LNP) Conjugation Methods
Post-intercalation Method
The most common and reliable method. A ligand pre-conjugated with polyethylene glycol-lipid is incubated with pre-formed lipid nanoparticles (LNPs), and the ligand spontaneously intercalates into the outer monolayer.
Pre-conjugation Coupling Method
Functional groups are coupled to lipids prior to LNP assembly. This method can provide higher density but requires careful optimization to avoid interfering with the self-assembly process.
Surface Coupling Method
Functional groups are directly chemically coupled to active groups on the surface of a pre-formed LNP (e.g., using DBCO-azide "click" chemistry on functionalized lipids).
GTOnco™ Platform Advantages and Highlights
One-stop Service
From lipid synthesis to in vivo validation.
Strict Quality Control
Research-grade raw materials compliant with cGMP standards.
IP Protection
Comprehensive agreements ensure your research results are not infringed.
Flexibility & Scalability
From milligram-level research batches to gram-level preclinical studies.
Customer Review
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Dr. E.K.
Chief Scientific Officer
“Creative Biolabs’ expertise in siRNA-LNP conjugation has been crucial in advancing our liver fibrosis drug candidate. Their detailed characterization reports and reliable scale-up protocols based on SPAAC chemistry saved us months of in-house optimization time. The final product exhibited the targeted in vivo properties we needed, with excellent batch-to-batch consistency.”
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Professor A.T.
Principal Investigator
“The transition from research-grade to preclinical LNPs was very smooth, thanks to the successful progress of our cancer immunotherapy program. Their team excelled in site-specific antibody conjugation on the LNP surface, ultimately obtaining a highly homogeneous product with significantly improved binding affinity to tumor cells compared to our initial in-house development.”
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Ms. R.C.
Head of Drug Development
“We presented Creative Biolabs with a very challenging requirement: to develop a dual-ligand LNP functionalized material capable of crossing the blood-brain barrier. They were able to integrate two different conjugation chemistry approaches—thiol-maleimide for targeting and polyethylene glycolation for improved stability—into a single LNP. The surface reveals a level of chemical technology we've never seen before. Currently, the product is progressing smoothly through all the studies required for the IND application.”
Frequently Asked Questions
For initial feasibility and optimization runs, we typically require 1-5 mg of the purified ligand. This allows for rigorous HTS screening of stoichiometric ratios and reaction conditions. For production batches, the requirement is calculated based on the target LNP batch size and the desired ligand density.
An optimized, mild conjugation process is designed to occur exclusively on the LNP surface and should not negatively impact the integrity or encapsulation efficiency of the internal cargo. We rigorously test for cargo leakage and LNP dissociation using HPLC and RiboGreen assays immediately after conjugation and purification to ensure high stability is maintained.
Ligand density is a critical parameter for optimal targeting. We use specialized, quantitative assays—such as ELISA for antibody ligands, or fluorometric/mass spectrometric quantification for peptides/small molecules—run against a known standard curve to precisely determine the amount of conjugated ligand per milligram of lipid. This is expressed as the molar ratio of ligand to total lipid (L : L).
Our development and scale-up services follow stringent GMP-like principles (strict SOPs, documented batch records, high-level QC) suitable for preclinical and IND-enabling studies. While we do not perform full commercial GMP manufacturing in-house, we can seamlessly transition successful protocols and technology to a qualified, client-selected GMP manufacturing partner.
Yes, we offer multi-ligand conjugation. This "multi-valent" targeting can enhance specificity and avidity. This is a complex service that we design and validate on a case-by-case basis.
We require your payload (if any) and the functional ligand (antibody, peptide sequence, etc.). We can also source or synthesize these for you through our integrated services.
Connect with Us Anytime!
LNP conjugation services are no longer just an auxiliary step, but a key strategic element in realizing the full potential of next-generation targeted therapies and vaccines. By precisely covalently attaching specific functional ligands to the LNP surface, Creative Biolabs transforms universal delivery vectors into highly functionalized, customized smart drug systems, thereby significantly improving targeting, stability, and therapeutic efficacy.
for a quotation or any question
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