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siRNA In Vitro Screening Service


In order to identify and validate the targets of interest, researchers use siRNA collections with readouts to take full advantage of the potential of siRNAs present for drug discovery. Creative Biolabs now provides convenient siRNA in vitro screening services. Adding siRNA screening to your early drug discovery screening programs enables customers to identify targets that traditional biochemical and cell-based screening & profiling may miss. Our sophisticated technologies and professional scientists are available to provide you with customized siRNA screening services.

siRNA In Vitro Screening Introduction

The whole process of siRNA in vitro screening service is shown in Figure 1. Creative Biolabs provides appropriate scale of RNAi library to screen (e.g., genome wide, kinase family or customer-defined) and develops robust and reproducible assays for identification and selection of candidate genes. Meanwhile, Creative Biolabs will choose the appropriate type of RNAi library, including chemically synthesized siRNAs, short hairpin siRNA (shRNA) and endoribonuclease-derived siRNAs (esiRNA), based on your genes.

Flowchart of RNAi high-throughput screening. Figure 1. Flowchart of RNAi high-throughput screening. (Gao, 2014)

There are two formats of siRNA in vitro screening services: a pooled screening format, in which the library is introduced randomly into cells (Figure 2a), or an arrayed screening format, in which single genes are targeted by reagents in individual wells of a microtiter plate (Figure 2b).

Two formats of siRNA in vitro screening. Figure 2. Two formats of siRNA in vitro screening. (Mohr, 2010)

Arrayed Screening

Pooled Screening

The processes of the positive screen and negative screen. Figure 3. The processes of the positive screen and negative screen.

Creative Biolabs aspires to become the trusted solution provider of your first choice. Our advanced technologies and highly experienced staffs are committed to advancing your program with our siRNA in vitro screening services and reducing the overall development timeline. If you have any special requirements in the siRNA screening services, please don’t hesitate to contact us.

References

  1. Gao, S.; et al. (2014). Applications of RNA interference high-throughput screening technology in cancer biology and virology. Protein & Cell. 5(11): 805-815.
  2. Mohr, S.; et al. (2010). Genomic screening with RNAi: results and challenges. Annual review of biochemistry. 79: 37-64.

For Lab Research Use Only, Not for Human or Animal Therapeutic Use.

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