Introduction of GJA8
Gap junction alpha-8 protein (GJA8), known as connexin 50 (Cx50), is a protein which in humans is encoded by the GJA8 gene. Cx50 is a connexin isoform of 50 kDa. The GJA8 gene locates on human chromosome 1. GJA8 is associated with maintaining lens opacity and proper lens development. Impaired GJA8 could lead to small eye problems, small mirror size and cataracts.
|Basic Information of GJA8|
|Protein Name||Gap junction alpha-8 protein|
|Organism||Homo sapiens (Human)|
Function of GJA8 Membrane Protein
GJA8 has been shown to form functional hemichannels and promote lens fiber differentiation in vitro, independent of its channel forming ability. Gap-junction channels allow direct exchange of ions and small signal/metabolism molecules between adjacent cells and play a key role in many physiological processes. GJA8 is found in the cytoplasm and nucleus of glial cells, astrocytes, and oligodendrocyte-derived cells. Similar expression patterns were also found in the primary culture of mature astrocytes. Furthermore, when epSPC and activated epSPCi were used to differentiate into mature oligodendrocytes, the opposite expression profile of nuclear GJA8 was observed, suggesting that this ion channel has a different role in the spinal cord during intercellular communication. GJA8 was detected in vivo by immunohistochemistry, showing a defined position in the gray matter of non-lesional tissues.
Fig.1 The membrane topology of Gap junction alpha-8 protein. (Beyer, 2013)
Application of GJA8 Membrane Protein in Literature
The results demonstrate that the mutation of GJA8 in heterozygous state affects significantly the lipid profile and the oxidative stress parameters in the spontaneously hypertensive rat strain.
This article reveals that loss of Cx50-mediated coupling, BS disruption, and altered F-actin in GJA8 KO fibers contribute to the small lens and mild cataract phenotypes.
These data indicate that the pore surface charges at the TM1/E1 border domain of GJA8 channel are crucial for the γj and Vj gating properties.
The results suggest a minor or detrimental contribution of GJA8 in spinal cord injury regeneration.
MD simulation revealed that the introduction of the deletion destabilized the GJA8 gap junction channel, indicating the deletion as a dominant-negative mutation.
GJA8 Preparation Options
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