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GJB2 Membrane Protein Introduction

Introduction of GJB2

Gap junction beta-2 protein (GJB2) is a protein encoded by the GJB2 gene. It is also known as connexin-26(cx26). GJB2 is involved in the movement of small metabolites and ions between the neighboring cells. The gap junction is first characterized by an electron microscope and has a regional specific structure on the plasma membrane contacting the attached cells. These structures have been shown to consist of cells to cell channels. Proteins purified from the rich gap junctions of different tissues, called connexins, are different. Connexins are specified by their molecular mass. Another nomenclature divides connexins into two categories: alpha and beta, based on sequence similarity at nucleotide and amino acid levels.

Basic Information of GJB2
Protein Name Gap junction beta-2 protein
Gene Name GJB2
Aliases Connexin-26
Organism Homo sapiens (Human)
UniProt ID P29033
Transmembrane Times 4
Length (aa) 226
Sequence MDWGTLQTILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQADFVCNTLQPGCKNVCYDHYFPISHIRLWALQLIFVSTPALLVAMHVAYRRHEKKRKFIKGEIKSEFKDIEEIKTQKVRIEGSLWWTYTSSIFFRVIFEAAFMYVFYVMYDGFSMQRLVKCNAWPCPNTVDCFVSRPTEKTVFTVFMIAVSGICILLNVTELCYLLIRYCSGKSKKPV

Function of GJB2 Membrane Protein

Mutation of the GJB2 gene-encoded protein Cx26 in homozygous and heterozygous states is an important part of the recessive form of chromosomes in different populations. This genetic defect leads to the most common congenital deafness in developed countries, called DFNB1 (also known as connexin 26 deafness or gjb2-related deafness). Through the regulation of the cell cycle, GJB2 also plays an important role in tumor suppression. Abnormal expression of GJB2, associated with several types of human cancer, may be a prognostic factor for cancers such as colorectal cancer, breast cancer, and bladder cancer. In addition, it is recommended to promote the development of cancer by promoting cell migration and invasion, and to promote the development of cancer by stimulating the self-renewal capacity of cancer stem cells.

Fig.1 The structure of Gap junction beta-2 protein.

Application of GJB2 Membrane Protein in Literature

  1. Holzer L.A., et al. Expression of Gap Junction Proteins Connexins 26, 30, and 43 in Dupuytren’s Disease. Acta Orthopaedica. 2014, 85 (1): 97–101. PubMed ID: 24359029

    The results show that GJB2 has high expression in α-SMA positive myofibroblast-rich nodules, indicating that connexins could be a novel treatment target for the treatment of DD.

  2. Chandrasekhar A., et al. Intercellular Redistribution of cAMP Underlies Selective Suppression of Cancer Cell Growth by Connexin26. PLoS ONE. 2013, 8(12): e82335. PubMed ID: 24312655

    This article implies that GJB2 inhibit transformed growth of HeLa cells.

  3. Palmer C.G.S., et al. Impact of Genetic Counseling and Connexin-26 and Connexin-30 Testing on Deaf Identity and Comprehension of Genetic Test Results in a Sample of Deaf Adults: A Prospective, Longitudinal Study. PLoS ONE. 2014, 9 (11): e111512. PubMed ID: 25375116

    The results suggest that the GJB2 and Connexin-30 genes are intimately connected to Deaf culture.

  4. Fiori M.C., et al. Functional Hemichannels Formed by Human Connexin 26 Expressed in Bacteria. Bioscience Reports. 2015, 35 (2): e00177. PubMed ID: 25585383

    The previous researches indicate that mutations of Cx26 (connexin 26) are the most frequent cause of genetic deafness.

  5. Polusani S.R., et al. Cell Coupling Mediated by Connexin 26 Selectively Contributes to Reduced Adhesivity and Increased Migration. Journal of Cell Science. 2016,129 (23): 4399–4410. PubMed ID: 27777264

    This article indicates that expression of Cx26 in HeLa cells selectively enhances wound healing in a manner that is dependent on intercellular coupling.

GJB2 Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-GJB2 antibody development services.


Creative Biolabs' skillful scientists are glad to leverage our expertise and advanced technologies to help you with the member protein research. If you are interested, please feel free to contact us for more details.

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