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Cardiolipin is an indispensable phospholipid primarily found in the inner membrane of the mitochondria, the cell's energy-generating organelle. In mammals, it is generated through a pathway involving cardiolipin synthase acting on glycerol and cytidinediphosphate-diacylglycerol. This lipid is critical for maintaining mitochondrial integrity and dynamics, helping to control the cellular commitment to programmed cell death, and serving as a proton sink essential for respiratory chain function.