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Anti-Lewis Antigen Antibody Development Services

Overview

Lewis antigens are carbohydrate determinants in the histo-blood group system and tumor-associated glycans. They are formed through the action of glycosyltransferases such as FUT2 and FUT3, with structural variations influenced by genetic background and secretor status. Clinically, Lewis antigens—including Lewis A, Lewis B, Lewis X, Lewis Y, and their sialylated or modified variants—play critical roles in transfusion medicine, pregnancy serology, oncology, and infectious disease. These glycans are increasingly recognized as valuable biomarkers and therapeutic targets, highlighting the importance of precise and reproducible antibody development.

Are you currently facing challenges with unreliable serological tools, inconsistent glycan-specific detection, or difficulties in developing antibodies against complex carbohydrate antigens? Anti-Lewis antigen antibody development services at Creative Biolabs help you generate highly specific and reliable antibodies through advanced immunogen design, chemoenzymatic glycan synthesis, and validated high-throughput screening platforms.

We offer comprehensive antibody development across the Lewis family of glycans:

  • Core antigens: Lewis A, Lewis B, Lewis X, Lewis Y, Lewis M, Lewis N.
  • Sialylated forms: Sialyl-Lewis A, Sialyl-Lewis B, Sialyl-Lewis X, Sialyl-Lewis Y, Sialyl-Lewis M.
  • Modified variants: Sulfo Sialyl-Lewis X, Non-Fucosylated Lewis X, and Fucosyl-Lewis X.

Strategies we applied:

  • Chemoenzymatic synthesis of Lewis A, B, X, Y, M, N, and their sialylated, fucosylated, and sulfated derivatives for accurate immunogen preparation.
  • Pathway-informed antibody development using well-characterized glycosyltransferases to ensure physiological epitope representation.
  • Specialized targeting of rare modifications such as sulfo sialyl-Lewis X, non-fucosylated Lewis X, and fucosyl-Lewis X.
  • Validation through glycan arrays, ELISA, and SPR to confirm high specificity and reduce cross-reactivity.

Learn How We Can Accelerate Your Research – Request a Consultation Today.

Service Portfolio

Anti-Lewis A Antibody Development

Custom antibody generation targeting Lewis A, a type I chain glycan widely studied in transfusion medicine and tumor biomarker research.

Anti-Lewis B Antibody Development

Development of antibodies against Lewis B, supporting clinical serology, host–pathogen interaction studies, and glycan-based diagnostics.

Anti-Lewis X Antibody Development

Production of highly specific antibodies recognizing Lewis X, an oncofetal antigen linked to cancer progression and developmental biology.

Anti-Lewis Y Antibody Development

Antibody design for Lewis Y, a tumor-associated carbohydrate antigen applied in oncology research, diagnostics, and therapeutic development.

Anti-Lewis M Antibody Development

Custom antibody services for Lewis M, supporting research into rare blood group variants and transfusion compatibility testing.

Anti-Lewis N Antibody Development

Generation of antibodies against Lewis N, providing tools for advanced serological studies and niche clinical research applications.

Anti-Sialyl-Lewis A Antibody Development

Creation of antibodies targeting sialyl-Lewis A, a key cancer biomarker involved in metastasis and widely used in oncology diagnostics.

Anti-Sialyl-Lewis B Antibody Development

Antibody development for sialyl-Lewis B, enabling research into gastrointestinal cancers and glycan-mediated infection pathways.

Anti-Sialyl-Lewis X Antibody Development

Production of antibodies against sialyl-Lewis X, an essential ligand for selectins, supporting studies of inflammation and metastasis.

Anti-Sialyl-Lewis Y Antibody Development

Generation of antibodies specific to sialyl-Lewis Y, a promising target in cancer immunotherapy and diagnostic development.

Anti-Sialyl-Lewis M Antibody Development

Design of antibodies against sialyl-Lewis M, offering specialized tools for rare antigen profiling and comparative glycan analysis.

Anti-Sulfo Sialyl-Lewis X Antibody Development

Custom antibody development for sulfo sialyl-Lewis X, critical in immunology and inflammation research as a selectin-binding glycan.

Anti-Non-Fucosylated Lewis X Antibody Development

Production of antibodies recognizing non-fucosylated Lewis X, enabling fine discrimination of glycan modifications in research and diagnostics.

Anti-Fucosyl-Lewis X Antibody Development

Development of antibodies against fucosyl-Lewis X, supporting tumor biology research and assays for glycan-structure-specific functions.

What We Can Offer

Custom Antibody Development

We design and generate monoclonal, recombinant, and humanized antibodies specifically tailored to Lewis antigens, ensuring optimal specificity and reproducibility.

Chain-Length Sensitive Solutions

Our team provides antibody panels with sensitivity to glycan structures and subtle modifications, enabling accurate profiling across diverse applications.

Multipurpose Validation

Every antibody is validated across multiple applications such as IHC, IF, WB, and flow cytometry, allowing seamless integration into your workflow.

Comprehensive Analytical Support

We supply clients with detailed characterization data, including epitope recognition profiles, binding kinetics, and cross-reactivity analysis, ensuring each antibody performs reliably in complex biological samples.

Workflow

01Project Consultation

Define target antigen subtype (e.g., Lewis B, Sialyl-Lewis X) and intended application. Clarify project scope, assay formats, and deliverables to ensure the strategy aligns with client goals.

02Immunogen Design

Generate authentic glycan structures through chemoenzymatic synthesis, with precise control of linkages and modifications. Conjugate to carriers like BSA or KLH, and confirm accuracy using MS or HPLC.

03Antibody Generation

Apply hybridoma or phage display platforms to produce high-affinity antibodies. Screen diverse isotypes and subclones to build a strong candidate pool.

04Screening & Validation

Test candidates with glycan microarrays, ELISA, and inhibition assays to confirm specificity. Cross-check against related glycans to minimize off-target binding.

05Optimization

Refine performance through affinity maturation and isotype engineering, enhancing binding strength, stability, and reproducibility across applications.

06Final Characterization

Provide datasets covering specificity, sensitivity, kinetics, and cross-reactivity, with recommendations for use in IHC, flow cytometry, or diagnostic ELISA.

Required Starting Materials

  • Defined antigen of interest (e.g., Lewis A, sulfo sialyl-Lewis X).
  • Intended application (oncology biomarker research, transfusion testing, infection studies).

Highlights


Expertise in Glycobiology
Creative Biolabs offers deep expertise in Lewis antigen pathways, ensuring precise targeting across complex glycan families.

Robust Glycan Synthesis
Chemoenzymatic synthesis provides high-purity, structurally defined immunogens for consistent antibody generation.

Coverage of Rare Variants
Unlike generic solutions, our service includes rare epitopes such as sulfo and non-fucosylated Lewis X.

Discover the Creative Biolabs Advantage – Contact Us for a Customized Quote.

Customer Reviews

Expanded Biomarker Coverage
“Using Creative Biolabs’ Anti-Sialyl-Lewis A antibody in our oncology research significantly improved tumor progression tracking with high sensitivity.” [Cancer Researcher, Dr. K***]

Reliable Serology Tools
“Creative Biolabs developed a custom Anti-Lewis B antibody that enhanced our transfusion compatibility studies and eliminated inconsistencies seen with commercial reagents.” [Clinical Scientist, Dr. W***]

Unique Epitope Targeting
“The Anti-Sulfo Sialyl-Lewis X antibody from Creative Biolabs enabled our team to explore inflammatory pathways that were previously inaccessible.” [Immunologist, Dr. P***]

FAQs

Which Lewis antigens can you target?

We support antibody development for classical Lewis antigens, sialylated derivatives, and modified forms, including sulfo and fucosylated variants.

Are rare antigens like non-fucosylated Lewis X included?

Yes, our synthesis platforms allow the design of rare immunogens for precise antibody development.

How do your services compare to off-the-shelf antibodies?

Our antibodies are customized, validated, and optimized for application-specific needs, outperforming generic commercial clones.

Extended Services

Custom Glycan Synthesis Services

We provide tailor-made synthesis of glycans with defined structures, linkages, and degrees of polymerization. Using chemoenzymatic and chemical strategies, we deliver high-purity glycans for use in immunogen preparation, biomarker discovery, and functional studies.

Glycan Modification and Labeling Services

We offer precise glycan modification and labeling, including biotinylation, fluorescent tagging, and carrier conjugation. These services enable sensitive detection, assay integration, and advanced applications such as imaging, glycan arrays, and diagnostic development.

Creative Biolabs’ Anti-Lewis Antigen Antibody Development Services provide precise detection tools for cancer biomarkers, transfusion research, inflammatory disease models, and pathogen adhesion studies. To explore your project needs in detail, Contact Our Team today.

For Research Use Only.

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