Antibody Glycoprofiling Service

Antibody Glycoprofiling Service at Creative Biolabs

The glycosylation pattern of an antibody is a key quality attribute, which has an important impact on the structure, safety, stability, and immunogenicity of the antibody. Comprehensive glycosylation analysis of antibodies helps evaluate the impact of different glycosyl structures on antibody functions and activity. Creative Biolabs has developed a multifunctional Glycoprotein Analysis Platform. We use various mass spectrometry techniques to comprehensively identify the glycoforms of antibodies and conduct professional glycosylation profile assessments of Fab and Fc domains.

Glycoprofiling analysis of antibodies is generally performed according to the following steps.

• Sample preparation

We collect and extract antibody samples, and perform protein purification and enrichment processing on the samples to reduce the impact of interfering substances on glycosylation analysis and eliminate non-specific signals.

• Enzymatic digestion reaction

We add an appropriate enzyme (such as PNGase F) to the antibody sample and incubate it for several hours at 37 °C to remove glycosylation. The released glycans are then labeled.

• Chromatographic separation

We use liquid chromatography technology or capillary electrophoresis to elute and separate labeled glycans and calculate the relative glycan composition.

• Mass spectrometry (MS) analysis

To correctly identify the labeled glycans, we couple the separation system to an MS system and use positive ion mode to collect precursor ions and fragment product ions of the sample. Moreover, we analyze the glycan composition in the antibody sample and generate detailed reports according to the detected mass spectrometry data, including glycan type, relative abundance, possible modification types, etc., thereby providing clients with comprehensive analysis results on the antibody glycans.

Fig.1 The workflow of antibody glycoprofiling.

Publication

Technology: Capillary electrophoresis-mass spectrometry (CE-ESI-MS)

Journal: Talanta

IF: 6.556

Published: 2018

Results: The authors used CE-ESI-MS technology to comprehensively characterize the N-glycosylation of monoclonal antibodies (mAbs) and relative quantification of the glycosylation profiles of mAbs produced in different cell lines was performed. The results showed that the CE-ESI-MS method had good reproducibility and stability and could be used as an alternative to liquid chromatography-mass spectrometry (LC-ESI-MS) for glycosylation analysis.

Fig.2 CE-ESI-MS technology for antibody glycoprofiling.¹

Advantages

• We provide glycoprofiling for various biological samples, such as antibodies, Serum, Cell Tissues, Urine, etc.
• We have established a complete standardized operating process to ensure the accuracy and reliability of the glycoprofiling analysis process.
• We promptly respond to client needs and provide efficient sample processing and glycoprofiling analysis services.

Application

• Biological stability assessment: Analyzing the glycosyl structure in antibody samples evaluates the impact of glycosyl on the biological stability and half-life of the antibody in vivo, helping to optimize the stability of the antibody.
• Pharmaceutical research: Analysis of the glycosyl structure of antibodies helps to understand the pharmacodynamic properties of antibodies, including its impact on receptor binding affinity and activity, as well as the impact of glycosyl on the metabolism and clearance rate of antibodies in the body.
• Drug development: Antibody glycosylation analysis provides important information for drug development, which is used to help optimize antibody drug design.

Creative Biolabs has experienced scientific research teams dedicated to providing global clients with a wide range of antibody glycoprofiling analyses. Whether you use glycoprofiling for research, development, or quality control, you can trust us to provide quality glycoprofiling services tailored to your specific needs. Please feel free to contact us if you are interested in our glycoprofiling analysis.

Reference

1. Giorgetti, Jérémie, et al. "Monoclonal antibody N-glycosylation profiling using capillary electrophoresis–Mass spectrometry: Assessment and method validation." Talanta 178 (2018): 530-537.

Related Services:

• Glycoprotein Detection
• Glycoprotein Structure Analysis
• Glycoprotein Quantification
• High-throughput Glycan Screening
• Glycosylation Analysis for Virus Glyoprotein
• Serum Glycoprofiling
• Plasma Glycoprofiling
• Tumor Tissue Glycoprofiling
• Tumor Cell Line Glycoprofiling
• Urine Glycoprofiling
• Quantitative Sialic Acid Analysis
• Quantitative Monosaccharaide Analysis
• Sugar Nucleotide Analysis
• Glycosaminoglycan (GAG) Analysis