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This material is a water-soluble equivalent of phosphatidylinositol 3-phosphate (PI(3)P), with biotin attached at the sn-1 site. Phosphoinositides are integral signaling molecules that, despite their minor concentration in the lipid bilayer, play essential roles in directing cellular processes. PI(3)P acts as a definitive membrane marker for early endosomal and multivesicular compartments. Its primary cellular functions encompass governing endosome fusion events, mediating the precise sorting of internalized receptors, and trafficking cargo.