Carbohydrate Analysis Services
Carbohydrates play key roles in countless biological processes, but the analysis of them is intricate. With a variety of advanced technologies, Creative Biolabs offers a range of services in carbohydrate analysis. With a variety of advanced technologies, we are confident in solving any puzzle in carbohydrate analysis for customers.
Introduction of Carbohydrates
Carbohydrates are found virtually everywhere in living systems and play key roles in countless biological processes. Their best-known functions are as energy resources, as a backbone element in the DNA and RNA genetic molecules, and as builders of structural bio-scaffoldings in plants and insects. However, their biological activity is more diverse. Carbohydrates are central actors in cell signaling, molecular recognition, immunity, and inflammation. Fruits of some plants contain high concentrations of glucose, fructose, and raffinose-family oligosaccharides such as sucrose, whereas in bacterial cell walls complex glycans composed of glucosamine and muramic acid, and sometimes derivatives of higher members (heptose and octose) of monosaccharides are found. In animal tissues and body fluids, there are various kinds of carbohydrates including mono-, oligo-, and polysaccharides, which play important roles in supporting the living activity of animals. Glucose is one of the major constitutes of blood and generates energy when metabolized in the tricarboxylic acid cycle (TCA cycle). Abnormal increase of glucose level in blood is a signal of diabetes, and therefore the rapid estimation of its level is an important problem in clinical chemistry. Some kinds of sea animals are covered by hard shells that are built up by the polymerized glucosamine. Carbohydrates exist in animal bodies not only in the unconjugated state but also in various kinds of glycoconjugates.
Carbohydrate Analysis Service at Creative Biolabs
Advantages of Our Service
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Professional: Creative Biolabs has years of experience in carbohydrate analysis service.
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Reliable: Creative Biolabs has developed a high-quality platform with advanced technologies and the latest methods.
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Customers-first: Creative Biolabs provides efficient, accurate, and cost-efficient services.
As a leading CRO company, Creative Biolabs has developed a cutting-edge platform with advanced technologies and experienced scientists. We are pleased to meet every specific technical or research requirement of our global customers. Please feel free to contact us for more information.
Published data
The date juice contains monosaccharides, disaccharides, vitamins, and mineral salts. Sugars are one of the main carbon sources in many fermentation processes and are widely applied in different food processing technologies. Therefore, it is important to analyze the sugar content in date juice. This study aims to develop and optimize a method for analyzing the sugar content in food. First, the authors used the standards of common sugars in date juice (such as glucose, fructose, and sucrose) to prepare the corresponding standard sugar solutions. The date juice samples were dried, ultrasonicated, vortexed, and centrifuged to get the supernatant to be analyzed. Then, the prepared standard sugar solution and the sample supernatant to be analyzed were subjected to oximation and silylation derivatization reactions, and the reaction products were sent to gas chromatography-mass spectrometry (GC-MS) for analysis. The results showed that fructose (3.882min and 3.905min corresponding to the positive and negative configurations of fructose), glucose (3.950min and 3.990min corresponding to the cis and trans forms of glucose), and sucrose (5.330min) could be detected in the date juice samples. Each hexose showed two peaks, while sucrose had only one peak. In addition, characteristic fragment ion peaks of various sugars were observed in the mass spectra of the date juice samples, and these sugars were quickly identified by MS technology. This study showed that the GC-MS-based analysis method could quickly identify and quantify various sugar components in date juice, with fast analysis speed and high sensitivity.
Fig.1 Total ion chromatogram (TIC) of date juice sample after oximation-silylation.1
FAQs
Q1: What are the main technologies Creative Biolabs uses for monosaccharide and oligosaccharide analysis?
A1: We utilize a variety of advanced technologies for monosaccharide and oligosaccharide analysis, including:
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HPAE-PAD: It is ideal for separating and quantifying monosaccharides. PAD detects carbohydrates without pre-column derivatization, and the detection is both efficient and sensitive, capable of detecting picomolar amounts.
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CE: When analyzing monosaccharides by CE, the sample is separated by electrophoresis, where they move at different speeds within a charged capillary tube according to their charge/size ratio.
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HPLC: It is very effective in separating and identifying complex carbohydrate mixtures.
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MS: This technique provides detailed information on the molecular weight and structure of carbohydrates.
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FACE: Labeled monosaccharides are separated by size and charge migration speed under the action of an electric field. Subsequently, individual monosaccharides are quantitatively detected using a fluorescence detector, thereby achieving qualitative and quantitative analysis of monosaccharides.
Q2: How do you customize protocols for carbohydrate analysis projects?
A2: Creative Biolabs' experienced scientists work closely with clients around the world to understand their specific research requirements and goals. Based on this information, we customize protocols, including selecting the appropriate analysis technique, optimizing experimental conditions, and designing appropriate control conditions to ensure accuracy and reliability. This customized approach ensures that the analysis meets the unique needs of each project.
Q3: How do you determine the molecular weight of polysaccharides?
A3: For polysaccharide molecular weight determination, Creative Biolabs combines multiple analysis techniques to resolve the complexity of these molecules. Typically, we use gel permeation chromatography (GPC) or size exclusion chromatography (SEC) combined with multi-angle light scattering (MALS) and refractive index (RI) detectors. These techniques help determine the molecular weight and molecular weight distribution of polysaccharides. In addition, the use of MS provides complementary data for detailed structural elucidation.
Customer Review
Solving Clients' Experimental Problems
"Our research team has been working on the analysis of the sugar structure of a special pathogen's cell wall, but due to its complex structure, it is difficult to obtain ideal results using conventional methods. Creative Biolabs' expert team uses high-resolution UHPLC/FLD/Q-TOF technology to not only help us analyze the polysaccharide composition of the cell wall but also provide an important basis for subsequent drug development."
Unique Technical Advantages
"Creative Biolabs has unique technical advantages in sugar analysis. Their HPAE-PAD and MS combined technology not only improves detection sensitivity but also effectively separates and quantifies complex carbohydrate samples. No matter how complex the sugar composition is, it can not stump them, which greatly improves our research efficiency."
Reference
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Al-Mhanna, Najah M., Holger Huebner, and Rainer Buchholz. "Analysis of the sugar content in food products by using gas chromatography mass spectrometry and enzymatic methods." Foods 7.11 (2018): 185. Distributed under Open Access license CC BY 4.0, without modification.
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