Our Products Introduction Glycosylation Overexpression Cell Lines Glycosylation Knockout Cell Lines Advantages FAQs Related Services
Glycoengineered Cells: Overexpression & Knockout Solutions
Looking for effective tools to explore the role of glycosylation in biological systems? Creative Biolabs offers a diverse range of glycoengineered cell lines using precise overexpression and knockout techniques. Our specially glycan-modified cell lines, which include CHO, 293 cells, and around 800 tumor-derived lines, offer valuable opportunities to study the changes in glycosylation that may affect a variety of diseases. These include, but are not limited to, cancers such as melanoma, bladder urothelial carcinoma, conditions like acute myeloid leukemia (AML), and meningothelial tumors. Using these glycoengineered cells, researchers are empowered to meticulously dissect the functional sequelae arising from precisely defined glycosylation modifications, capitalizing on a dual offering of both immediately accessible and bespoke, custom-engineered cellular models tailored to specific research inquiries.
Deciphering Glycosylation Functionality: Overexpression of Cellular Models
These cell lines exhibit increased expression of certain glycosyltransferases or other enzymes critical to glycan biosynthesis. This allows for the study of the functional impact of increased levels of specific glycans on cellular behavior, signaling pathways, and interactions with other molecules.
Applications include:
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Investigating the role of specific glycans in receptor signaling and ligand binding.
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Analyzing the impact of hyper-glycosylation on protein stability and function.
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Developing cell-based assays to screen for modulators of specific glycosylation pathways.
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Studying the effects of increased glycan expression on immune recognition and evasion.
Fig.1 Illustration of key segments of the N-glycosylation pathway in murine myeloma cell lines and CHO cells.1
Unlock Glycosylation Insights with Our Glycosylation Knockout Cell Lines
These cell lines have specific glycosylation-related genes knocked out, leading to the absence or significant reduction of particular glycan structures. This enables the direct assessment of the functional roles of these glycans. Applications include:
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Assessing the role of specific glycans in crucial cellular processes such as adhesion and migration.
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Identifying the contribution of individual glycans to disease pathogenesis.
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Developing targeted therapies that exploit specific glycosylation vulnerabilities.
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Creating cell models with simplified glycosylation profiles for easier analysis.
Advantages of Choosing Creative Biolabs' Glycoengineered Cell Products
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Extensive and Diverse Collection: Access a broad range of cell types, including our comprehensive library of approximately 800 tumor-derived cell lines relevant to diverse disease areas.
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Precisely Engineered Models: Our cell lines are generated using advanced gene editing techniques, ensuring accurate and stable modifications of target genes.
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Ready-to-Use Formats: Our characterized cell lines are delivered ready for immediate integration into your experimental workflows, saving you valuable time and resources.
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Stringent Quality Control: Each cell line undergoes rigorous testing to confirm the intended genetic modification and ensure cell viability and stability.
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Expert Scientific Support: Our team of experienced glycobiologists and cell engineers provides comprehensive support to help you select the optimal cell lines and experimental strategies for your research.
Frequently Asked Questions (FAQs)
Q: What types of overexpression cell lines do you offer?
A: We provide a range of overexpression cell lines, including those with increased expression of glycosyltransferases, glycosidases, and other proteins that play a role in glycan synthesis and modification. If you're looking for a specific target, don't hesitate to get in touch with us.
Q: How are the knockout cell lines made?
A: Our knockout cell lines are made using advanced gene editing tools like CRISPR-Cas9, which allow us to precisely knock out the target gene.
Q: Can I request a specific overexpression or knockout cell line that's not in your catalog?
A: Yes! We offer custom glycoengineering services. Just let us know the specific details of the cell line and modification you need, and we'll be happy to assist you in creating it.
Q: How are the overexpression and knockout modifications validated?
A: We employ a range of molecular biology techniques, such as PCR, Western blotting, flow cytometry, and NGS, to confirm the genetic modification and its impact on protein expression. Glycan analysis can also be performed to verify changes in glycosylation profiles.
Related Services
Except for our overexpression and knockout glycoengineered cell products, Creative Biolabs offers comprehensive glycoengineering services to further support your research in glycobiology and glyco-chemistry, including but not limited to:
Contact us today to discuss how our glycoengineered cell solutions can accelerate your research endeavors.
Reference
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Gupta, Shivani, et al. "Engineering protein glycosylation in CHO cells to be highly similar to murine host cells." Frontiers in Bioengineering and Biotechnology 11 (2023): 1113994. Distributed under Open Access license CC BY 4.0, without modification.
