Tag based Glycan Library Products

Comprehensive Tagged Glycan Reference Libraries: Accelerating Glycomics Research

Glycosylation is not merely a common post-translational modification, which is a critical quality attribute in biopharmaceuticals and a dynamic regulator of cellular signaling, immune response, and pathogen interaction. The inherent structural complexity of glycans involves numerous monosaccharide types, linkages, and branching patterns, resulting in immense microheterogeneity, posing significant analytical challenges. To navigate this complexity and meet stringent regulatory requirements, modern glycoanalysis demands tools that ensure both ultra-sensitivity and absolute structural certainty. Creative Biolabs provides the indispensable tag-based glycan library tools for modern glycomics. We offer an extensive range of chemically labeled, highly purified Glycan Library standards, synthesized or isolated and characterized using advanced techniques. These products are manufactured to rigorous specifications, ensuring the unparalleled precision and traceability required for robust method validation, reliable absolute quantification, precise structural confirmation, and definitive system suitability testing in the most demanding research, development, and quality control environments.

Chemical labeling, which involves labeling the free reducing ends of released glycan molecules, is the cornerstone of high-performance glycan analysis. This process, typically achieved through highly efficient reductive amination, covalently links the glycan to a specialized tag. By introducing a fluorescent or charged moiety, the tag serves two critical and complementary functions:

Various Glycan Tag Structures

Our reference libraries are built upon a versatile, quality-driven platform. Each standard undergoes a stringent analytical assessment to guarantee integrity before chemical tagging and final purification. The fundamental structure is a glycan molecule linked covalently to a fluorescent tag at the reducing terminus, creating a stable, traceable, and highly detectable chemical complex ready for analysis. All glycan standards are subjected to multi-modal quality control testing, including:

• Purity assessment: Typically via chromatography or electrophoresis to confirm high purity and integrity.
• Structural confirmation: Verification via MS to confirm the mass and sequence.
• Absolute quantification: Accurate concentration determination using techniques like quantitative fluorescence spectroscopy, ensuring precise mass balance.

Table 1 The chemical category of the key label.

Whether your analytical platform is mass spectrometry, chromatography, or electrophoresis, we ensure you have the right labeled standards for clear, definitive, and reliable detection and separation of the most complex glycan mixtures.

Comprehensive Product Range and Glycan Diversity

Our libraries span the full spectrum of biologically and pharmaceutically relevant glycan structures, ensuring a suitable reference standard for nearly any glycoanalytical need. We provide a variety of glycan products, both labeled and unlabeled.

• N-Glycan Standards

N-linked glycans are central to the efficacy and safety of monoclonal antibodies (mAbs) and other therapeutic glycoproteins. Our standards are crucial for characterizing biotherapeutic molecules and complex tissue samples.

• O-Glycan Standards

O-linked glycosylation, which commonly starts with an N-acetylgalactosamine (GalNAc) attachment, is pivotal in mucins, growth factor receptors, and signaling molecules.

• Other Monosaccharide & Antigenic Standards

Beyond complex chains, we provide labeled standards for monosaccharides, antigenic structures, and non-mammalian epitopes to support specialized linkage and composition analysis.

Key Application

Our tag-based glycan library is seamlessly integrated into every essential stage of modern analytical science, accelerating validation and ensuring compliance.

Analytical Method Validation

Establishing and documenting critical method performance characteristics, including column performance, assay linearity, precision (repeatability and intermediate precision), specificity, and robustness. It is essential for meeting strict regulatory expectations.

Quantification and Bioprocess Monitoring

Providing accurate, traceable calibration curves for the reliable measurement of glycan quantities in unknown samples, enabling optimized cell culture conditions and consistent manufacturing.

Structural Assignment and Confirmation

Co-injection of standards with unknown sample glycans provides unambiguous confirmation of identity and highly reproducible retention time comparison, acting as an essential primary evidence source.

Enzyme Characterization and Functional Assays

Used as specific substrates or positive/negative controls to verify the activity, reaction kinetics, and linkage specificity of glycosidase and glycosyltransferase enzymes, critical for both research and industrial deglycosylation protocols.

Biomarker Discovery

Reference standards enable the precise quantification of disease-associated glycan biomarkers in clinical samples, providing the necessary benchmark for developing sensitive diagnostic assays.

Pharmacokinetic/Pharmacodynamic (PK/PD) Studies

Used to track subtle changes in drug glycosylation in vivo and understand how these changes impact the drug's half-life and mechanism of action.

Take the Next Step in Your Glycomics Journey: Partner with Creative Biolabs

Achieving reliable, traceable, and reproducible glycan data begins with high-quality, fully characterized reference standards. Our comprehensive library, featuring a variety of essential N- and O-glycan structures paired with high-performance tags (2-AB, APTS, procainamide), provides the fundamental accuracy and confidence you need to advance your research. Please inquire today to speak with our technical specialists about customizing a reference library solution and optimizing your current analytical methods. Let us be your partner in glycan precision.