As the trusted partner for leading pharmaceutical and biotech companies worldwide, Creative Biolabs' success is built on a foundation of deep scientific expertise, a commitment to cutting-edge technology, and a passion for solving our clients' most complex challenges. When you choose to work with us, you gain more than a service provider. You gain a dedicated team of scientific partners who are personally invested in your project's success. We take pride in our flexible and collaborative approach. We listen to your unique needs and design a custom service package that aligns perfectly with your goals, your timelines, and your budget.
Service Structure & Workflow
Our Custom Glycosylation Services are designed as a modular yet integrated framework. You can engage with one or more of the sub-services depending on your project needs. Here’s a high-level workflow overview:
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Project initiation and need‑analysis
We begin by understanding your target glycoprotein, production system (including cell line, expression vector, and host species), analytical history, project timelines, and regulatory requirements. Our technical team will help define key glyco‑attributes (e.g., glycan type, site occupancy, branching, sialylation level) and propose the appropriate strategy.
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Glycosylation analysis (baseline profiling)
If not already available, we perform comprehensive glycan mapping and site-specific profiling to establish the “glyco-fingerprint” of your molecule. This provides the baseline against which engineering changes or comparability adjustments can be evaluated.
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Glycosylation engineering (design and implementation)
Based on the baseline and project goals, we apply engineering tools, including cell line optimization, glycosyltransferase/glycosidase modulation, and glycan remodeling to generate glycoprotein variants with defined glycosylation patterns.
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Glycosylation characterization
Engineered variants are assessed for glycan composition, site occupancy, structural consistency, batch-to-batch variation, functional activity, and biophysical stability. This ensures that the glyco‑modifications are not only present but compatible with the molecule’s function and manufacture.
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Deliverables and interpretation
We provide comprehensive reports, including raw data files, interpretation of glyco-modifications, and recommendations for next steps. We also hold follow-up consultations to support your decision-making.
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Glycosylation testing(applications)
Following structural characterization and data delivery, we can advance your project into functional validation for research applications. This stage applies your engineered glycoproteins in specific application-focused assays. Key areas include evaluating their utility as glycosylated protein biomarkers for discovery and assay development, as well as assessing their biological function in research models related to glycosylation disorders.
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Optional iterative optimization
If required, additional rounds of engineering and testing can be performed to refine glyco-profiles. Our service is flexible to accommodate iterative development campaigns.
The ability to combine all phases under one roof, including analysis, engineering, characterization, and functional testing, provides a streamlined workflow, reduced handoffs, consistent documentation, and improved traceability from initial glyco-design decisions to final application validation.
Our Comprehensive Service Offerings
At Creative Biolabs, we are dedicated to providing innovative and customized solutions tailored to your specific glycosylation-related needs. With several years of experience, we have refined our service offerings to help you navigate the complexities of glycosylation analysis, engineering, and testing. Whether you're developing therapeutics, improving drug delivery, or exploring glycoprotein biomarkers, we are here to support your success every step of the way. Discover how our tailored services can meet your unique requirements.
We use state-of-the-art techniques to characterize and profile glycans, enabling you to understand their role in biological activity, stability, and efficacy.
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N-Glycosylation Analysis
Let us assist you in identifying and quantifying N-linked glycans in proteins, providing the precision you need for the development of therapeutic glycoproteins. Our N-glycosylation analysis service is designed to give you a deeper understanding of glycan structures and their implications for your research and drug development.
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O-Glycosylation Analysis
Our O-glycosylation analysis service goes beyond simple glycan identification, helping you analyze the structure and function of O-linked glycans. Understanding these structures is crucial for ensuring your glycoproteins remain stable and effective, particularly in complex therapeutic environments.
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DNA Glycosylase and GlycoRNA Analysis
Our DNA glycosylase and glycoRNA analysis service. This service helps uncover the role of glycans in gene regulation and cellular functions, which is invaluable for basic research.
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Fc Region Glycosylation Analysis
Understand how glycosylation influences the function of antibodies with our Fc region glycosylation analysis service. This service is designed to optimize monoclonal antibody therapies, improving their effector functions, stability, and immune response profiles.
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Specialized Glycoconjugate Analysis
Whether you're working on glycoconjugate-based drugs or complex biomolecules, our specialized glycoconjugate analysis service will help you unlock the biological roles of glycans in diverse systems, pushing the boundaries of novel therapeutic molecules.
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High-Throughput Glycomic Analysis
For large-scale glycosylation studies, our high-throughput glycomic analysis service offers cutting-edge technology to analyze complex glycosylation patterns quickly and accurately, ideal for high-volume applications in research and development.
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Glycosylation Inhibition
Our glycosylation inhibition services help you understand the effects of inhibiting glycosylation pathways. This service is particularly crucial for drug discovery and cancer research, providing valuable insights that can inform the development of new therapeutic strategies.
Take control of your glycosylation profiles with our glycosylation engineering platform. Designed for lab research, our platform allows precise modifications of glycan structures to enhance the functionality of your molecules.
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Glycosylation of Expressed Proteins/Peptides
By optimizing the glycosylation of recombinant proteins and peptides, we can enhance their stability, efficacy, and immunogenicity. Let us help you improve the quality and performance of your expressed glycoproteins, ensuring they meet the demands of your therapeutic or research goals.
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Glycosylated Antibody Engineering
Transform your antibody therapeutics with our glycosylated antibody engineering service. We develop antibodies with tailored glycosylation profiles, enhancing their pharmacokinetics, efficacy, and immune responses, making them better suited for clinical use.
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Glycolipid Engineering
Glycolipids play an essential role in cellular signaling and membrane functions. Our glycolipid engineering services allow you to modify lipid-linked glycans to enhance cell targeting, stability, and overall therapeutic performance.
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Glycosylated Oligonucleotide Engineering
Improve the stability and delivery of nucleotides with our glycosylated oligonucleotide engineering services. By incorporating glycans into the structure of oligonucleotides, we can enhance their bioavailability and therapeutic potential.
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Glycosylation of Small Molecule Drugs
Optimize the glycosylation of small molecules to improve their bioavailability and efficacy. Our services are tailored to enhance drug delivery applications, ensuring that your small molecule drugs perform at their best.
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Glycosylated Polymer Engineering
Our glycosylated polymer engineering services help improve the encapsulation, targeting, and controlled release of drug payloads, which is especially important for the development of advanced nanomedicine applications.
Ensure your glycoproteins perform optimally with our Glycosylation Testing Services. We assess the structural and functional impacts of glycosylation modifications, ensuring that your molecules meet the necessary standards for both efficacy and manufacturability.
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Glycosylation Disorder Solutions
For researchers focused on glycosylation disorders, we offer customized solutions to address these diseases. Our expertise supports the development of therapies targeting glycan-related diseases, bringing you closer to breakthrough solutions.
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Glycosylated Protein Biomarkers
Leverage our glycosylated protein biomarker services to identify and validate glycosylation-based biomarkers for disease diagnosis and therapeutic monitoring. These biomarkers can provide critical insights into disease progression and treatment response.
How to Start Your Project
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Submit the inquiry form on this page with the requested project information (target molecule, host system, current glyco‑data, project timeline).
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We will schedule a technical consultation within 24–48 hours to refine your scope.
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You receive a detailed proposal including workflow phases, deliverables, timeline, and cost estimate.
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Upon agreement, we initiate the project and assign a dedicated project manager to track milestones, update you regularly, and deliver a clear deliverable package.
Fig.1 How to start your custom glycosylation project with Creative Biolabs?
Why Choose Creative Biolabs for Custom Glycosylation Services
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End-to-end support: From initial glycan mapping to engineered manufacture and final testing, we offer complete lifecycle support.
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Speed and flexibility: We understand development timelines; our workflows are optimized for rapid turnaround without sacrificing quality.
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Actionable results: Beyond delivering data, we interpret the implications for your molecule, linking glycan alterations to function, manufacturability, and regulatory impact.
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Partner mindset: Your success is our priority; we communicate clearly, adapt dynamically, and integrate smoothly with your development team.
Typical Use‑Cases We Address
To give you a clearer sense of how our custom glycosylation capabilities are applied, here are common scenarios we support:
Therapeutic monoclonal antibody optimization
A developer identified that their candidate antibody exhibited sub‑optimal effector function due to heterogeneous Fc glycosylation (e.g., elevated high‑mannose or afucosylated species). Through our tailored glycosylation engineering platform, we generated a variant enriched in fucosylated, fully sialylated structures, followed by functional testing and glycan profiling via our analysis service. The result: improved ADCC/CDC profile and reduced clearance risk.
Enzyme glycan‑remodeling
An enzyme intended for lysosomal delivery required a mannose-6-phosphate tag and controlled terminal sialylation to enhance cellular uptake. Our team assessed the baseline glycan composition via analysis services, introduced glycosyltransferase modulation in the expression host, and confirmed via testing services that the engineered variant met the glyco-specification for research needs.
Biosimilar comparability and glycan matching
When submitting to regulatory authorities, matching the glycosylation pattern of the originator molecule is often a critical quality attribute. Our combined services enable you to map the originator’s glycofingerprint, engineer your biosimilar host accordingly, and test the manufactured batches to ensure glycan equivalence, thereby reducing the risk of regulatory review.
Biomarker development & disease‑glycomics
In biomarker research, altered glycosylation patterns serve as distinguishing features. We partner with you to perform comprehensive glycosylation profiling of disease-relevant targets, enabling you to define disease-specific glycoforms or assess glycoprotein heterogeneity as a biomarker.
At Creative Biolabs, we are dedicated to delivering the most advanced and reliable glycosylation services. From glycosylation analysis to glycosylation engineering and testing, our team is ready to support your projects and ensure the highest quality outcomes. Whether you are working on glycoproteins, antibodies, or novel therapeutic molecules, our comprehensive service offerings are tailored to help you succeed. Contact us today to explore how our customized glycosylation services can enhance your research and development.
FAQs
Do I have to purchase the entire workflow, or can I just use your glycosylation analysis service?
Our services are entirely modular. You can engage only with our analysis services to establish a baseline, or only use our engineering platform if you already have robust analytical data. We designed the workflow to be flexible, allowing you to select the specific phases—analysis, engineering, or testing—that align perfectly with your current needs.
Can you perform site-specific occupancy analysis, or only characterize the total released glycans?
We absolutely perform site-specific analysis. While total glycan pool profiling is a standard approach, our platform excels at glycopeptide mapping using high-resolution mass spectrometry. This allows us to determine the specific glycan structures present at each glycosylation site (macro- and micro-heterogeneity) and quantify site occupancy.
What is the typical turnaround time for a baseline glycan profiling project?
Timelines vary based on project complexity, such as the number of sites and the depth of analysis required. However, for a standard baseline profiling project, we typically aim to deliver the comprehensive data package and interpretation report within a few weeks. We will provide a specific timeline in your detailed project proposal.
Our mAb has high fucose levels and low ADCC. Can your engineering platform specifically address this?
Yes, this is a common application for our platform. We can utilize cell line optimization, such as modulating glycosyltransferase activity, to generate afucosylated or low-fucose antibody variants. We will then characterize these engineered variants to confirm the desired glycan profile, providing you with a molecule optimized for enhanced ADCC effector function.
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