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Cy5-Sphingosine is D-erythro-sphingosine labeled with the red-shifted Cy5 fluorophore. Sphingosine is a bioactive lipid base, acting as a precursor to other sphingolipids and serving as a critical regulator of cell fate decisions. This fluorescent probe is highly valuable for monitoring sphingolipid metabolism and investigating its involvement in signaling pathways that control cell differentiation, migration, and the induction of apoptosis.