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An indispensable internal standard for phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), featuring a stable deuterium label (D5) and a diC16 acyl chain. PI(4,5)P2 is an integral signaling molecule that regulates G-protein coupling and internal calcium stores. This labeled standard is crucial for the high-accuracy quantitative determination of endogenous PI(4,5)P2 levels in lipidomics assays via mass spectrometry.