Creative Biolabs is a fully equipped laboratory facility with specialized equipment for glycan analysis. With Ph.D. level scientists and extensive experience in applying gas chromatography-mass spectrometry (GC-MS) for analysis, we are happy to share our knowledge in this field to facilitate our customers’ project development.
Protein glycosylation is one of the major post-translational modifications (PTMs), which occurs in about 50% of proteins in humans. Synthesized without a template, glycans are key players in various significant intra- and extracellular events. Most glycans have complex structures and are comprised of a large number of monosaccharides linked by glycosidic bonds. The structure complexity is the result of various monosaccharides being attached to the protein via different linkages (N-linkage or O-linkage), linkage positions and branching.
Many pathological conditions affect gene expression, which can in turn deregulate the abundance of glycans. Unique aberrant glycans are often present in trace quantities within complex biological samples, making their detection difficult and sometimes stochastic. However, GC-MS, one of the oldest forms of mass spectrometry, can routinely be used to detect the collection of branch-point and linkage-specific monosaccharides present in complex samples. Mass spectra acquired by this hyphenated technique offers more structure-related information, and thus, monosaccharides can be characterized by GC-MS. In order to use GC-MS, hydrophilic monosaccharides need to be derived by trimethylsilylation or acetylation. GC-MS analyses of derivatized monosaccharides usually allow for an excellent chromatographic resolution for monosaccharides, thus providing structural information for identification.
Fig.1 Schematic diagram of GC-MS. (Kumar, 2015)
Creative Biolabs provides comprehensive GC-MS for glycan analysis services based on top-class scientific experts and substantial experience. Our instrument can detect pM amount of samples and is primarily used for monosaccharide and linkage analysis of glycans. Complementary to traditional glycomics techniques, GC-MS involves the collection and condensation of each constituent glycan in a sample into a single independent analytical signal, which provides detailed structural and quantitative information about changes to the glycome as a whole.
With proven experience in glycoscience, Creative Biolabs is an ideal company to entrust with your business. Our continually expanding range of instruments including GC-MS allows us to offer a wide range of glycan analysis services. We have perfected our technical pipelines of GC-MS for glycan analysis. For more detailed information, please feel free to contact us or directly send us an inquiry.