GlycoErase™ ACLY Knockout HepG2 Cell Line

GlycoErase™ ACLY knockout HepG2 cell line is a human liver-derived HepG2 model lacking ACLY expression. ATP citrate lyase (ACLY) generates cytosolic acetyl-CoA from citrate, linking energy metabolism with lipid and cholesterol biosynthesis. Loss of ACLY disrupts acetyl-CoA supply, impacting lipid metabolism, membrane formation, and downstream regulation. This knockout provides a human hepatic platform for studying acetyl-CoA-dependent pathways, metabolic flux, and lipogenesis, supporting research in metabolic disorders, therapeutic development, and liver disease modeling.

KO Cell Lines

Human

Epithelial-like, adherent

1:4~1:6

HepG2

Hepatoblastoma

Liver

Hepatoblastoma

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Glutamine

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ACLY

Atp Citrate Lyase

ACL; ATPCL; CLATP

17q21.2

ATP citrate lyase is the major enzyme responsible for producing cytosolic acetyl-CoA in numerous tissues. It exists as a tetramer of identical subunits with a total molecular weight of about 440 kDa. This enzyme converts citrate and CoA into acetyl-CoA and oxaloacetate, coupled with the hydrolysis of ATP to ADP and phosphate. The resulting acetyl-CoA feeds into critical biosynthetic pathways, such as fatty acid and cholesterol synthesis. In the nervous system, it may contribute to acetylcholine production. Several transcript variants encoding distinct isoforms have been identified.