GlycoErase™ AGL Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF492)

Description	GlycoErase™ AGL knockout HEK293T cell line is a HEK293T model in which AGL expression has been eliminated. AGL encodes the glycogen debranching enzyme, which carries both 4-alpha-glucotransferase and amylo-1,6-glucosidase activities, essential for glycogen breakdown. Deficiency of this enzyme is linked to glycogen storage disorders and metabolic variability across tissues. The knockout of AGL in the HEK293T background provides a specialized system for investigating glycogen metabolism, energy utilization, and carbohydrate processing, while supporting applications in metabolic disease research, therapeutic screening, and cell signaling studies.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	AGL
Full Name	Amylo-Alpha-1, 6-Glucosidase, 4-Alpha-Glucanotransferase
Alternative Name	GDE
Location	1p21.2
Gene ID	178
Summary	Glycogen debranching enzyme is involved in glycogen breakdown, with 4-alpha-glucotransferase and amylo-1,6-glucosidase activities. Mutations in this gene lead to glycogen storage disease, with clinical variability potentially due to alternative splicing in different tissues.