GlycoErase™ ALG10 Knockout Human Thyroid Carcinoma Cell 8305C (CAT#: GLJF-0525-JF641)

Description	The ALG10 deficient human thyroid carcinoma cell line 8305C serves as a crucial in vitro system for dissecting the intricacies of N-glycosylation; its deficiency in ALG10 leads to compromised N-glycan biosynthesis, consequently impacting protein folding fidelity, thus enabling researchers to probe the sequelae of N-glycosylation impairment in the context of thyroid carcinoma progression, metastatic potential, and drug sensitivity, as well as to identify potential diagnostic or therapeutic biomarkers.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Large spindle-shaped cell, adherent
Passage Ratio	1:3~1:6
Cell Line	8305C
Primary Disease	Anaplastic Thyroid Cancer
Lineage	Thyroid

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ALG10
Full Name	ALG10 alpha-1,2-glucosyltransferase
Alternative Name	ALG10A, DIE2, FLJ14751
Location	12p11.1
Gene ID	84920
Summary	This gene encodes a membrane-associated protein that plays a critical role in N-linked glycosylation by adding the third glucose residue to the lipid-linked oligosaccharide precursor. Specifically, it transfers the terminal glucose molecule from dolichyl phosphate glucose (Dol-P-Glc) to the lipid-linked oligosaccharide Glc₂Man₉GlcNAc₂-PP-Dol. This step is essential for the proper assembly of the oligosaccharide precursor necessary for N-linked glycoprotein synthesis.