GlycoErase™ ALG12 Knockout Human Malignant Melanoma Cell A375

The A375 cell line harboring a targeted disruption of the ALG12 gene represents a potent tool for dissecting the consequences of N-glycosylation impairment in malignant melanoma, as the resultant deficiency in ALG12 leads to the formation of aberrant N-glycan structures, thus allowing for the examination of how such glycosylation defects modulate tumor growth dynamics, interactions within the tumor microenvironment, the development of chemoresistance, and the rational design of targeted therapeutic interventions.

KO Cell Lines

Human

Epithelial-like, adherent

1:3~1:4

A375

Melanoma

Skin

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Glutamine

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ALG12

ALG12 alpha-1,6-mannosyltransferase

ECM39, CDG1G

22q13.33

This gene encodes a protein that belongs to the glycosyltransferase 22 family. The protein plays a crucial role in protein glycosylation by catalyzing the addition of the eighth mannose residue to the dolichol pyrophosphate-linked oligosaccharide precursor (dolichol-PP-Man₇GlcNAc₂). This addition occurs via an alpha-1,6 linkage, and is essential for the proper assembly of the oligosaccharide chain required for N-linked glycoprotein synthesis.