GlycoErase™ ALG5 Knockout Human Lung Cancer Cell A-427 (CAT#: GLJF-0525-JF1284)

Description	The ALG5 deficient A-427 cell line constitutes a significant model for analyzing the initial glucosylation steps in N-glycosylation, where the lack of ALG5 perturbs N-glycan assembly and protein folding, thereby allowing researchers to dissect the impact of these glycosylation deficiencies on lung adenocarcinoma cell survival, metastatic dissemination, interactions with the surrounding stroma, and the screening of compounds capable of modulating N-glycosylation pathways.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:3
Cell Line	A-427
Primary Disease	Non-Small Cell Lung Cancer
Lineage	Lung

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ALG5
Full Name	ALG5 dolichyl-phosphate Beta-glucosyltransferase
Alternative Name	bA421P11.2
Location	13q13.3
Gene ID	29880
Summary	Essential for oligomannose core glucosylation (N-linked); ensures substrate recognition, enabling efficient transfer to glycoproteins - critical for proper protein folding and function.