GlycoErase™ ALG5 Knockout HepG2 Cell Line (CAT#: GLJF-0425-JF1028)

Description	The ALG5 knockout HepG2 cell line is an important model for analyzing early glucosylation's impact on hepatic protein folding and function. Lacking ALG5 affects N-glycosylation and protein folding in HepG2 cells, potentially disrupting the quality control mechanisms for newly synthesized liver proteins. Researchers can analyze how these defects impact hepatoblastoma cell survival, potential metastasis within the liver, interactions with the liver microenvironment, and screen for N-glycosylation modulators with liver-specific effects.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ALG5
Full Name	ALG5 dolichyl-phosphate Beta-glucosyltransferase
Alternative Name	bA421P11.2
Location	13q13.3
Gene ID	29880
Summary	Essential for oligomannose core glucosylation (N-linked); ensures substrate recognition, enabling efficient transfer to glycoproteins - critical for proper protein folding and function.