GlycoErase™ ALG5 Knockout HepG2 Cell Line

The ALG5 knockout HepG2 cell line is an important model for analyzing early glucosylation's impact on hepatic protein folding and function. Lacking ALG5 affects N-glycosylation and protein folding in HepG2 cells, potentially disrupting the quality control mechanisms for newly synthesized liver proteins. Researchers can analyze how these defects impact hepatoblastoma cell survival, potential metastasis within the liver, interactions with the liver microenvironment, and screen for N-glycosylation modulators with liver-specific effects.

KO Cell Lines

Human

Epithelial-like, adherent

1:4~1:6

HepG2

Hepatoblastoma

Liver

Hepatoblastoma

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Glutamine

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ALG5

ALG5 dolichyl-phosphate Beta-glucosyltransferase

bA421P11.2

13q13.3

Essential for oligomannose core glucosylation (N-linked); ensures substrate recognition, enabling efficient transfer to glycoproteins - critical for proper protein folding and function.